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Shimazaki, Hiroko; Saito, Kozue; Matsuda, Atsushi; Sawakami, Kazumi; Kariya, Minoru; Segawa, Osamu; Miyashita, Yukiko; Ueda, Tetsuya; Koizuka, Michinori; Nakamura, Kazuhiro; Kaji, Hiroyuki; Tajima, Hideji; Kuno, Atsushi
Analytical chemistry (Washington), 09/2019, Volume: 91, Issue: 17Journal Article
A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omics-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of “bead arrays in a single tip.” The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan–lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.
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