The positive effects of trinexapac-ethyl (TE) on turfgrass growth and tolerance to various types of stress could promote its use in low-input maintenance turfgrasses to reduce the expense associated with mowing. However, there is a general lack of information about TE effects in low maintenance turfgrasses, and especially when used in transition zone turfgrass mixtures. The objective of this study was to evaluate the response of three inter-specific turfgrass mixtures to three different application rates of TE under low maintenance conditions. A 2-year field study was conducted from April 2006 to July 2008 at the Agricultural Experimental Farm of the University of Padova (northern Italy, 45°20′N, 11°57′E, elevation 8 m). Visual turfgrass quality (1–9 scale) and clippings biomass production of three mixtures were evaluated seasonally under four TE treatments with rates representing 0, 0.5, 1.0 or 1.5x the rates indicated on label instructions. Based on both visual quality and biomass production, turfgrass responded differently to TE application depending on dominant species in the mixture. Furthermore, our results suggested that changes over time in turfgrass botanical composition influence visual quality and biomass production through the experimental period. The main effect of TE treatments occurred in biomass production while the effect on turfgrass quality was mainly associated with the two highest TE rates. Application at a rate lower than the labeled seems to be enough for reducing biomass production without affecting turfgrass quality or even, in some seasons, improving it. Finally, we found that the effect of TE seems to weaken over time as turfgrass maturity advances. •There is few information about Trinexapac-ethyl use in low-input turfgrass mixtures.•Trinexapac-ethyl effect depends on dominant species in the mixture.•Trinexapac-ethyl affects biomass production more than visual quality.•Half the labeled rate is enough for reducing biomass production.