E-resources
-
Clark, Iain C; Gutiérrez-Vázquez, Cristina; Wheeler, Michael A; Li, Zhaorong; Rothhammer, Veit; Linnerbauer, Mathias; Sanmarco, Liliana M; Guo, Lydia; Blain, Manon; Zandee, Stephanie E J; Chao, Chun-Cheih; Batterman, Katelyn V; Schwabenland, Marius; Lotfy, Peter; Tejeda-Velarde, Amalia; Hewson, Patrick; Manganeli Polonio, Carolina; Shultis, Michael W; Salem, Yasmin; Tjon, Emily C; Fonseca-Castro, Pedro H; Borucki, Davis M; Alves de Lima, Kalil; Plasencia, Agustin; Abate, Adam R; Rosene, Douglas L; Hodgetts, Kevin J; Prinz, Marco; Antel, Jack P; Prat, Alexandre; Quintana, Francisco J
Science (American Association for the Advancement of Science), 04/2021, Volume: 372, Issue: 6540Journal Article
Cell-cell interactions control the physiology and pathology of the central nervous system (CNS). To study astrocyte cell interactions in vivo, we developed rabies barcode interaction detection followed by sequencing (RABID-seq), which combines barcoded viral tracing and single-cell RNA sequencing (scRNA-seq). Using RABID-seq, we identified axon guidance molecules as candidate mediators of microglia-astrocyte interactions that promote CNS pathology in experimental autoimmune encephalomyelitis (EAE) and, potentially, multiple sclerosis (MS). In vivo cell-specific genetic perturbation EAE studies, in vitro systems, and the analysis of MS scRNA-seq datasets and CNS tissue established that Sema4D and Ephrin-B3 expressed in microglia control astrocyte responses via PlexinB2 and EphB3, respectively. Furthermore, a CNS-penetrant EphB3 inhibitor suppressed astrocyte and microglia proinflammatory responses and ameliorated EAE. In summary, RABID-seq identified microglia-astrocyte interactions and candidate therapeutic targets.
Author
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.