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Rivas, Felipe; Erxleben, Dorothea; Smith, Ian; Rahbar, Elaheh; DeAngelis, Paul L; Cowman, Mary K; Hall, Adam R
American Journal of Physiology: Cell Physiology, 04/2022, Volume: 322, Issue: 4Journal Article
The carbohydrate hyaluronan (or hyaluronic acid, HA) is found in all human tissues and biofluids where it has wide-ranging functions in health and disease that are dictated by both its abundance and size. Consequently, hyaluronan evaluation in physiological samples has significant translational potential. Although the analytical tools and techniques for probing other biomolecules such as proteins and nucleic acids have become standard approaches in biochemistry, those available for investigating hyaluronan are less well established. In this review, we survey methods related to the assessment of native hyaluronan in biological specimens, including protocols for separating it from biological matrices and technologies for determining its concentration and molecular weight.
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