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Murakami, Toshiharu; Nakazawa, Tsutomu; Natsume, Atsushi; Nishimura, Fumihiko; Nakamura, Mitsutoshi; Matsuda, Ryosuke; Omoto, Koji; Tanaka, Yoshitaka; Shida, Youichi; Park, Young-Soo; Motoyama, Yasushi; Nakagawa, Ichiro; Yamada, Shuichi; Tamura, Kentaro; Takeshima, Yasuhiro; Takamura, Yoshiaki; Wakabayashi, Toshihiko; Nakase, Hiroyuki
Anticancer Research/Anticancer research, 09/2018, Volume: 38, Issue: 9Journal Article
Natural killer (NK) cells are considered potential antitumor effector cells. The aim of this study was to establish a novel type of a chimeric antigen receptor (CAR) NK cell line (CAR-KHYG-1) specific for epidermal growth factor receptor variant III (EGFRvIII)-expressing tumors and investigate the anti-tumor activity of EGFRvIII-specific-CAR-KHYG-1 (EvCAR-KHYG-1). EvCAR-KHYG-1 was established by self-inactivated lentiviral-based transduction of the EvCAR gene and magnetic bead-based purification of EvCAR-expressing NK cells. The anti-tumor effects of EvCAR-KHYG-1 were evaluated using growth inhibition and apoptosis detection assays in glioblastoma (GBM) cell lines (EGFRvIII-expressing and non-expressing U87MG). The findings demonstrated that EvCAR-KHYG-1 inhibited GBM cell-growth via apoptosis in an EGFRvIII-expressing specific manner. This is the first study to establish a CAR NK cell line based on the human NK cell line KHYG-1. Therapy with EvCAR-KHYG-1 may be an effective treatment option for GBM patients.
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