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Woldeyes, Mahlet A; Qi, Wei; Razinkov, Vladimir I; Furst, Eric M; Roberts, Christopher J
Journal of pharmaceutical sciences 108, Issue: 1Journal Article
Protein-protein interactions (PPI) and solution viscosities were measured at low and high protein concentrations under a range of formulation conditions for 4 different monoclonal antibodies. Static light scattering was used to quantify the osmotic second virial coefficient (B ) and the zero-q limit static structure factor (S ), versus protein concentration (c ) from low to high c . Dynamic light scattering was used to measure the collective diffusion coefficient as a function of c and to determine the protein interaction parameter (k ). Static light scattering and dynamic light scattering were combined to determine the hydrodynamic factor (H ), which accounts for changes in hydrodynamic PPI as a function of c . The net PPI ranged from strongly repulsive to attractive interactions, via changes in buffer pH, ionic strength, and choice of monoclonal antibodies. Multiple-particle tracking microrheology and capillary viscometery were used to measure monoclonal antibodies solution viscosities under the same solution conditions. In most cases, even large and qualitative changes in PPI did not result in significant changes in protein solution viscosity. This highlights the complex nature of PPI and how they influence protein solution viscosity and raises questions as to the validity of using experimental PPI metrics such as k or B as predictors of high viscosity.
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