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Tibbetts, R; Frye, J.G; Marschall, J; Warren, D; Dunne, W
Journal of Clinical Microbiology, 09/2008, Volume: 46, Issue: 9Journal Article
An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the blaKPC carbapenemase gene produced an amplicon of the expected size which was confirmed to be blaKPC₋₂ by sequence analysis. Transformation of a susceptible Escherichia coli host with plasmid preparations from the isolate generated a transformant for which the MICs of all of the carbapenems tested were increased three- to fourfold. We believe this to be the first report of carbapenem resistance in P. mirabilis caused by the acquisition of blaKPC.
