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Ogino, C; Kanemasu, M; Hayashi, Y; Kondo, A; Shimizu, N; Tokuyama, S; Tahara, Y; Kuroda, S; Tanizawa, K; Fukuda, H
Applied microbiology and biotechnology, 06/2004, Volume: 64, Issue: 6Journal Article
The structural gene for phospholipase D (PLD) of an actinomycete, Streptoverticillium cinnamoneum, together with its promoter region was introduced into Streptomyces lividans using a shuttle vector--pUC702--for Escherichia coli and S. lividans. The transformant was found to secrete a large amount of PLD (about 2.0x10⁴ U/l, 42 mg/l) when cultured in a jar fermentor. Both an initial glucose concentration of 17.5 g/l and the feeding of carbon and nitrogen sources are effective for efficient secretion of PLD; under these culture conditions, the amount of PLD secreted reached a maximum level (about 5.5 x10⁴ U/l, 118 mg/l) after about 60 h. In contrast to the original producer, Stv. cinnamoneum, which secretes only a small amount of PLD (about 1.1x10³ U/l, 2 mg/l) along with other extracellular proteins, this heterologous expression system is markedly more efficient in production of secretory PLD.
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