The effects of fertiliser application at the rates of P30K60 (treatment PK), N90P30K60 (treatment N90) and N180P30K60 (treatment N180) on changes in the botanical composition of seminatural grassland in a region of the Nizke Tatry mountains (The Low Tatras), Slovakia were investigated over 8 experimental years. Afterwards fertilizing was discontinued and the frequency of cutting was reduced from 3 to 2 cuts. The two-year stoppage of PK fertilizer application resulted in a legume proportion decrease from 41 to 27%. Consequently, the proportion of grasses in the sward increased by 12% during the two years. More substantial changes in botanical groups in the sward previously fertilized with N(+PK) were observed in treatment N180. Two years after the cessation of fertilizer application, only a slight decline (7%) in the proportion of grasses and a 5% increase in that of legumes were found in treatment N90. In treatment N180, however, a 20% decline in the proportion of grasses and a 16% increase in that of other herbs were recorded, and a larger area of bare ground was observed.