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Deza, C; Pérez, G.T; Gardenal, C.N; Varela, L; Villar, M; Rubiales, S; Barioglio, C
Small ruminant research, 03/2000, Volume: 35, Issue: 3Journal Article
Under the name of Creole goats, individuals of various origins, morphological and productivity characters are grouped. To characterize the genetic pool of goats from different sources in central Argentina, protein polymorphism revealed by electrophoresis was analyzed. A total of 109 adult animals from Santa Marı́a, Colón and Ischilı́n Districts, Córdoba Province, were studied. The red cells lysates were subjected to starch gel electrophoresis and specific staining procedures to reveal the activity of different enzymes and hemoglobin. Separation of plasmatic proteins was carried out by electrophoresis in polyacrylamide gel and stained with Coomassie Blue. From a total of 14 loci analyzed, eight were polymorphic in at least one flock. Four codominant alleles were detected in the locus βHemoglobin (βHb); three alleles in malic enzyme (Me), glucose 6 phosphate dehydrogenase (G6pdh) and lactate dehydrogenase (Ldh) and two alleles in catalase (Cat), transferrin (Tf), esterase-2 (Es-2) and phosphoglucomutase (Pgm). In all the cases, the observed genotype frequencies were not significantly different from those expected from Hardy–Weinberg equilibrium. The proportion of polymorphic loci ( P%) varied between 21.4% and 42.9% and mean heterozygosity ( H), between 0.061 and 0.117. Alleles at loci Cat, Me and Es-2 showed significantly different frequencies according to the sample origin. The flock from Colón District presented the highest levels of polymorphism. This is the only stock which includes hybrids of varying grade between Creole stocks and male specimens from Saanen and Nubian breeds.
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