Display omitted •Wheat straw-based itaconic acid is a promising alternative in biorefinery approach.•Pretreatment and fermentation stages account for the largest environmental burdens.•A renewable electricity has a significant influence on the environmental profile. This study performs the environmental assessment of itaconic acid (IA) production from wheat straw. The Life Cycle Assessment (LCA) methodology is used to determine the environmental hotspots, considering impact categories such as Global Warming (GW), Fossil Resource Scarcity (FRS), Water Consumption (WC), among others. A sensitivity analysis was performed considering an optimization of the steam explosion process and 100% renewable energy. Results report an impact of about 14.33 kg CO2 eq in GW, 4.15 kg of oil eq in FRS, for each kg of IA produced for the baseline scenario. Moreover, the pretreatment and fermentation stages constitute hotspots of the IA production. In addition, using a renewable energy source in production would reduce the impact by 82% in GW, 71% in PM and 82% in FRS categories. The optimization of the steam explosion process presents a better performance in GW and FRS but also lies in an increase in WC.