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Valli, M.B.; Bertolini, L.; Iacovacci, S.; Ponzetto, A.; Carloni, G.
Research in virology (Paris), 07/1995, Volume: 146, Issue: 4Journal Article, Conference Proceeding
The TOFE lymphoid cell line from normal human bone marrow is susceptible to infection by a hepatitis C virus (HCV) serum strain. A sequence analysis of the 5′ untranslated region (UTR) of HCV before and after long-term in vitro infection revealed one base substitution at position −158 (C > T) of the 5′ UTR. We performed the direct sequencing of 5′ UTR polymerase chain reaction-amplified sequences of the HCV genome: a) from the original serum-derived strain; b) from TOFE cell extracts 6 months post infection. This base substitution in the regulatory elements of the 5′ UTR might be related to the ability of the virus to grow in cell culture.
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