Biohydrogen production using renewable sources has been regarded as one of the most sustainable ways to develop low-cost and green production technology. In order to achieve this objective, herein biohydrogen production has been conducted using the combination of untreated secondary sewage sludge (Sss), algal biomass hydrolyzate (Abh), graphene oxide (GO) and bacterial consortia that forms a granular system. Thus, naturally formed granular system produced cumulative H2 of 1520 mL/L in 168 h with the maximum production rate of 13.4 mL/L/h in 96 h at initial pH 7.0, and optimum temperature of 37 °C. It is noticed that the combination of Abh, Sss and GO governed medium showed 42.05 % higher cumulative H2 production along with 22.71 % higher production rate as compared to Abh and Sss based H2 production medium. The strategy presented herein may find potential applications for the low-cost biohydrogen production using waste biomasses including Sss and Abh. Display omitted •Aerobic granular sludge (AGS) is used to produce biohydrogen via dark fermentation.•AGS is based on sludge, algal biomass, bacterial consortia and graphene oxide (GO).•GO supported improved dark fermentative biohydrogen have been performed using AGS.•GO supported system showed 42.05 % higher cumulative H2 production compared to control.•Strategy may be highly potential to produce biohydrogen using waste biomass.