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Ren, Yan; Liu, Ying; Zhang, Wenming; Ran, Junfeng; Li, Li; Zhang, Zhidan
Electrophoresis, February 2024, 2024-Feb, 2024-02-00, 20240201, Volume: 45, Issue: 3-4Journal Article
The bamboo shoot of Pleioblastus amarus (Keng) Keng f. is a medicinal and edible resource in China. In this study, three separation techniques were applied to identify the primary and secondary metabolites component of P. amarus bamboo shoots, including sheathless capillary electrophoresis electrospray ionization–mass spectrometry (CESI–MS), reverse‐phase liquid chromatography–MS (RPLC–MS), and hydrophilic interaction liquid chromatography–MS (HILIC–MS). A total of 201 metabolites were identified by the three methods. Among those metabolites, 146 were identified by RPLC–MS, 85 were identified by HILIC–MS, and 46 were identified by sheathless CESI–MS. These methods were complementary and had a linear coefficient. CESI–MS presented advantages in the identification of isomers, high sensitivity, very low sample usage, and good detection of polar and nonpolar metabolites, showing its unique applications in food analysis and prospects in metabolic research.
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