Purpose The purpose of this study was to investigate the blood levels related to the bone mineral density by using the dual energy X-ray absorption for females before menopause and males younger than 50 years old. Subjects and Methods Between August 1, and September 15, 2013, the Z-score was measured in females before menopause and males younger than 50 years old using a bone mineral density measuring instrument. After the measurement, the subjects were classified into two groups, that is, the below expectations and within expectations groups. Next, we analyzed and compared the differences in age, body mass index, and blood levels between the 2 groups. Results The results showed a correlation of 0.212 for total protein, −0.317 for alanine aminotransferase, −0.199 for gamma-glutamyl transferase, −0.358 for alkaline phosphatase, 0.266 for uric acid, −0.313 for lactate dehydrogenase, 0.244 for creatinine, −0.234 for the red blood cell count, and −0.230 for the red cell distribution width in patients with less than expected level for their age. Conclusion In conclusion, osteoporosis may occur in females before menopause and males younger than 50 years old, and aggressive attention is required for prevention and treatment.