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Zhao, Z.W.; Chen, X.J.; Tay, B.K.; Chen, J.S.; Han, Z.J.; Khor, K.A.
Biosensors & bioelectronics, 08/2007, Volume: 23, Issue: 1Journal Article
ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 μA/mA cm 2. The low detection limit was estimated to be 20 μM (S/N = 3) and the apparent Michaelis–Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.
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