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Laojun, Sedthapong; Changbunjong, Tanasak; Chaiphongpachara, Tanawat
Insects, 01/2023, Volume: 14, Issue: 1Journal Article
There are four species of mosquitoes in Thailand, including , , , and The accurate species identification of adult mosquitoes based on morphological features requires many body parts, including the abdominal terga and wing. However, species identification is difficult in the case of damaged specimens when some of their morphological character is missing due to transit or gathering in the field. Thus, we evaluated the efficacy of the landmark-based geometric morphometric (GM) approach for the discrimination of species in Thailand. In addition, DNA barcoding was also used in parallel with the GM approach to identify the species. Larvae of were collected, raised into adults, and identified based on their morphological characteristics. The validated reclassification test results clearly demonstrated that wing shape resulted in a high level of success in identification (correct identifications ranged from 92.50% to 100%); however, based on the DNA barcoding analyses, our results showed that it was poorly effective in identifying and based on an overlap between the intraspecific and interspecific divergence. Moreover, our survey results provide updates on the distribution of and in Thailand. This research will help medical entomologists more efficiently identify mosquitoes in the genus , resulting in more effective mosquito control and surveillance.
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