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Choi, Yong Soo; Cho, Eun Sook; Je, Yeon Ho; Roh, Jong Yul; Chang, Jin Hee; Li, Ming Shun; Seo, Sook Jae; Sohn, Hung Dae; Jin, Byung Rae
Current microbiology, 01/2004, Volume: 48, Issue: 1Journal Article
Bacillus thuringiensis 656-3, isolated from a soil sample collected at mushroom houses, showed high toxicity to mushroom flies, Lycoriella mali and Coboldia fuscipes. B. thuringiensis 656-3 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis subsp. morrisoni (H8a8b). The plasmid and protein profiles of B. thuringiensis 656-3 were similar to those of its reference strain, subsp. morrisoni PG-14. However, PCR analysis using cry gene primers showed that B. thuringiensis 656-3, unlike its reference strain, had cry4A, cry4B, cry10A, cry11A, and cry1Ac genes, suggesting that B. thuringiensis 656-3 was a unique strain with respect to gene type. In addition, B. thuringiensis 656-3 showed a high level of toxicity against mushroom flies, L. mali and C. fuscipes. PUBLICATION ABSTRACT
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