Tooth loss has endangered human health for thousands of years, and people can apply dentures or dental implants to restore tooth loss today. Tissue engineering provides a novel way to regenerate a new functional tooth in vivo or vitro to help patients regain masticatory function and appearance. In this summarize review, we will discuss some promising seed cells in dental tissue engineering, the scaffolds that can be used to regenerate teeth, and some growth factors which can promote the development of tooth. Although significant progresses have been made nowadays, some challenges still remain. Hence, tissue engineering could be a choice to replace missing tooth in the future when the obstacles are solved.