E-resources
-
Kaniuk, Natalia A.; Canadien, Veronica; Bagshaw, Richard D.; Bakowski, Malina; Braun, Virginie; Landekic, Marija; Mitra, Shuvadeep; Huang, Ju; Heo, Won Do; Meyer, Tobias; Pelletier, Laurence; Andrews‐Polymenis, Helene; McClelland, Michael; Pawson, Tony; Grinstein, Sergio; Brumell, John H.
Cellular microbiology, November 2011, Volume: 13, Issue: 11Journal Article
Summary The facultative intracellular pathogen Salmonella enterica serovar Typhimurium establishes a replicative niche, the Salmonella‐containing vacuole (SCV), in host cells. Here we demonstrate that these bacteria exploit the function of Arl8B, an Arf family GTPase, during infection. Following infection, Arl8B localized to SCVs and to tubulated endosomes that extended along microtubules in the host cell cytoplasm. Arl8B+ tubules partially colocalized with LAMP1 and SCAMP3. Formation of LAMP1+ tubules (the Salmonella‐induced filaments phenotype; SIFs) required Arl8B expression. SIFs formation is known to require the activity of kinesin‐1. Here we find that Arl8B is required for kinesin‐1 recruitment to SCVs. We have previously shown that SCVs undergo centrifugal movement to the cell periphery at 24 h post infection and undergo cell‐to‐cell transfer to infect neighbouring cells, and that both phenotypes require kinesin‐1 activity. Here we demonstrate that Arl8B is required for migration of the SCV to the cell periphery 24 h after infection and for cell‐to‐cell transfer of bacteria to neighbouring cells. These results reveal a novel host factor co‐opted by S. Typhimurium to manipulate the host endocytic pathway and to promote the spread of infection within a host.
Author
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.