•Fertigation divided in two-times application promoted the photosynthesis parameters compared to a single fertilization and to the non-treated vines.•Via fertigation, biological and physiological activity of the whole plant was enhanced.•Fertigation tendentially and significantly improved the qualitative, productive, physiological, and oenological parameters of “Schioppettino” wine. Nowadays a balance in plant production is required, especially in terms of nutrition, yield and for an optimal aroma and sensory profile of resulted wines. In this research, we compared two different methods of plant nutrition: one-single application (single-fertilization-SF) and two applications with the same amount adopting the fertigation (split fertigation-SpF), including a control not-treated, in a specific Italian region where grapevine Schiopettino cv. is being cultivated and this practice was not investigated yet. SpF promoted the photosynthesis parameters compared to a SF and to the non-treated vines (NTC). On these basis, the biological and physiological activity of the whole plant was enhanced. SpF treatment tendentially and significantly improved the qualitative, productive, physiological, and oenological parameters of “Schioppettino” wine.