E-resources
-
Inoue, Masatoshi; Takeuchi, Atsuya; Manita, Satoshi; Horigane, Shin-ichiro; Sakamoto, Masayuki; Kawakami, Ryosuke; Yamaguchi, Kazushi; Otomo, Kouhei; Yokoyama, Hiroyuki; Kim, Ryang; Yokoyama, Tatsushi; Takemoto-Kimura, Sayaka; Abe, Manabu; Okamura, Michiko; Kondo, Yayoi; Quirin, Sean; Ramakrishnan, Charu; Imamura, Takeshi; Sakimura, Kenji; Nemoto, Tomomi; Kano, Masanobu; Fujii, Hajime; Deisseroth, Karl; Kitamura, Kazuo; Bito, Haruhiko
Cell, 05/2019, Volume: 177, Issue: 5Journal Article
To decipher dynamic brain information processing, current genetically encoded calcium indicators (GECIs) are limited in single action potential (AP) detection speed, combinatorial spectral compatibility, and two-photon imaging depth. To address this, here, we rationally engineered a next-generation quadricolor GECI suite, XCaMPs. Single AP detection was achieved within 3–10 ms of spike onset, enabling measurements of fast-spike trains in parvalbumin (PV)-positive interneurons in the barrel cortex in vivo and recording three distinct (two inhibitory and one excitatory) ensembles during pre-motion activity in freely moving mice. In vivo paired recording of pre- and postsynaptic firing revealed spatiotemporal constraints of dendritic inhibition in layer 1 in vivo, between axons of somatostatin (SST)-positive interneurons and apical tufts dendrites of excitatory pyramidal neurons. Finally, non-invasive, subcortical imaging using red XCaMP-R uncovered somatosensation-evoked persistent activity in hippocampal CA1 neurons. Thus, the XCaMPs offer a critical enhancement of solution space in studies of complex neuronal circuit dynamics. Display omitted Display omitted •Developing XCaMPs, a spectrally resolved, quadricolor (BGYR) suite of linear GECIs•Resolving PV interneuron spike trains and non-invasive imaging of hippocampal CA1•Cell-type-specific excitation-inhibition dynamics revealed by 3-color fiber photometry•Local dendritic inhibition identified in vivo via paired axon and dendrite recording Quadricolor suite of genetically encoded calcium indicators for multiplex recording in the brain.
Author
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.