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García-Cayuela, Tomás; de Cadiñanos, Luz P. Gómez; Mohedano, M. Luz; de Palencia, Pilar Fernández; Boden, Daniel; Wells, Jerry; Peláez, Carmen; López, Paloma; Requena, Teresa
Applied microbiology and biotechnology, 10/2012, Volume: 96, Issue: 1Journal Article
Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis , Enterococcus faecalis , and Escherichia coli .
