Reconstruction of cell wall polysaccharide is necessary for fruit development and ripening. Arabinose is the one of neutral sugars constituting wall polysaccharides and arabinose-containing polysaccharides play an important role in cellular attachment. In this study, expression patterns of α- l -arabinofuranosidase genes were examined in three tomato cultivars, the processing type ‘OSKAR’, fresh market type ‘Ailsa Craig’, and their hybrid ‘Shonan Pomoron Red’, to elucidate the function of α- l -arabinofuranosidase in fruit traits such as shape, growth, firmness, and juiciness. While there was no significant difference in fruit diameter among the cultivars, longitudinal elongation was observed in ‘OSKAR’ with increasing fruit weight. The hybrid ‘Shonan Pomoron Red’ showed intermediate longitudinal growth. Compared to ‘OSKAR’ at the red ripe stage, fruit flesh softened more in ‘Ailsa Craig’ and much water was released from the pericarp disk. The rate of water release in ‘Shonan Pomoron Red’ was intermediate. The α- l -arabinofuranosidase genes, SlArf/Xyl2, LeXYL1 and LeXYL2, were highly expressed in the young fruits and SlArf/Xyl1 and LeXYL1 were up-regulated when ripening initiated. However, their expression levels did not appear to cause the difference in fruit traits such as growth rate, fruit shape, and firmness among the cultivars. On the other hand, expression levels of SlArf/Xyl1, LeARF1 and LeXYL2 were consistent with the rate of water release from ripe fruit. Therefore, α- l -arabinofuranosidase could influence fruit flesh texture and juiciness in fresh market and processing cultivars.