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Preparation of three dimensional structures of polysaccharide derivatives for application in regenerative medicine : doctoral disertationDobaj-Štiglic, AndrejaBiocompatible polysaccharide scaffolds with controllable pore size, good mechanical properties, and no hazardous chemical crosslinkers are desirable for long-term tissue engineering applications. ... Despite decades of development of novel scaffolds, there are still many challenges to be solved regarding their production and optimization for specifically engineered tissues. Herein, we have fabricated several three-dimensional (3D) scaffolds using polysaccharide or polysaccharide-protein composite hydrogels or inks for 3D printing, featuring strong shear thinning behavior and adequate printability. The inks, composed of various combinations of chitosan, nanofibrillated cellulose, carboxymethyl cellulose, collagen, and citric acid, were 3D printed, freeze-dried, and dehydrothermally heat-treated to obtain dimensionally and mechanically stable scaffolds. The heat-assisted step induced the formation of covalent amide and ester bonds between the functional groups of chosen polysaccharides and protein collagen. Citric acid was chosen as a non-hazardous and „green” crosslinker to further tailor the mechanical properties and long-term stability of the scaffolds. We have investigated how the complexation conditions, charge ratio, dehydrothermal treatment, and degree of crosslinking influence the scaffolds' chemical, surface, swelling, and degradation properties in the dry and hydrated states. The compressive strength, elastic modulus, dimensional stability and shape recovery of the (crosslinked) scaffolds increased significantly with balanced charge ratio, dehydrothermal treatment, and increased concentrations of citric acid crosslinker and collagen concentrations. The prepared crosslinked scaffolds promoted (clustered) cell adhesion and showed no cytotoxic effects, as determined by cell viability assays and live/dead staining with human bone tissue-derived osteoblasts and human adipose tissue-derived mesenchymal stem cells. The water-based and non-hazardous crosslinking methods presented here can be extended to all polysaccharide- or polysaccharide-protein-based materials to develop cell-friendly scaffolds with tailored properties suitable for various tissue engineering applications.Vrsta gradiva - disertacija ; neleposlovje za odrasleZaložništvo in izdelava - Maribor : [A. Dobaj Štiglic], 2022Jezik - angleškiCOBISS.SI-ID - 125363971
Povezava(-e):
Digitalna knjižnica Univerze v Mariboru – DKUM
Digitalna knjižnica Slovenije - dLib.siDostop z namenskih računalnikov v prostorih NUK
Avtor
Dobaj-Štiglic, Andreja
Drugi avtorji
Stana-Kleinschek, Karin |
Maver, Uroš, 1983- |
Kargl, Rupert |
Maver, Tina
Teme
Tkivno inženirstvo |
Disertacije |
Univerzitetna in visokošolska dela |
hitozan |
karboksimetil celuloza |
nanofibrilna celuloza |
citronska kislina |
kolagen |
zamrzovanje s sušenjem |
3D tiskanje |
doktorske disertacije |
chitosan |
carboxymethyl cellulose |
nanofibrillated cellulose |
citric acid |
collagen |
freeze drying |
3D printing |
dehydrothermal treatment
Knjižnica/institucija |
Kraj | Akronim | Za izposojo | Druga zaloga |
---|---|---|---|---|
Knjižnica tehniških fakultet, Maribor | Maribor | KTFMB |
v čitalnico 1 izv.
|
|
Narodna in univerzitetna knjižnica, Ljubljana | Ljubljana | NUK |
v čitalnico 1 izv.
|
ni za izposojo 1 izv.
|
Univerzitetna knjižnica Maribor | Maribor | UKM |
v čitalnico 1 izv.
|
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Dobaj-Štiglic, Andreja | 34276 |
Stana-Kleinschek, Karin | 07814 |
Maver, Uroš, 1983- | 30850 |
Kargl, Rupert | 36303 |
Maver, Tina | 33260 |
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