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Synaptotagmin I increase the probability of vesicle fusion at low (Ca2+) in pituitary cellsKreft, Marko ...Synaptotagmin (Syt I), a low-affinity Ca2+ -binding protein, is thought to serve as the Ca2+ sensor in the release of neurotransmitter. However, functional studies on the calyx of Held synapse ... revealed that the rapid release of neurotransmitter requires only approximately micromolar šCa2+đ, suggesting that Syt I may play a more complex role in determining the high-affinity Ca2+ dependence of exocytosis. Here we tested this hypothesis bystudying pituitary cells, which possess high- and low-affinity Ca2+ -dependent exocytic pathways and express Syt I. Using patch-clamp capacitance measurements to monitor secretion and the acute antisense deletion of Syt I from differentiated cells, we have shown that the rapid and the most Ca2+ sensitive pathway of exocytosis in rat melanotrophs requires Syt I. Furthermore, stimulation of the Ca2+ -dependent exocytosis by cytosol dialysiswith solutions containing 1 micro M šCa2+đ was completely abolished inthe absence of Syt I. Similar results were obtained by the preinjection of antibodies against the CAPS (Ca2+ -dependent activator protein for secretion) protein. These results indicate that synaptotagmin I and CAPS proteins increase the probability of vesicle fusion at low cytosolic šCa2+đ.Vir: American journal of physiology. cell physiology. - ISSN 0363-6143 (Letn. 284, 2003, str. C547-54)Vrsta gradiva - članek, sestavni delLeto - 2003Jezik - angleškiCOBISS.SI-ID - 15875545
Avtor
Kreft, Marko |
Dominko, Asja |
Grilc, Sonja |
Rupnik, Marjan |
Milisav, Irina |
Zorec, Robert, 1958-
Teme
Pituitary Gland |
Exocytosis |
Calcium |
Calcium-Binding Proteins |
Rats, Wistar |
Patch-Clamp Techniques |
RNA, Messenger |
Polymerase Chain Reaction |
Hippocampus |
Cerebellum |
Spinal Cord |
Microscopy, Confocal |
Eksocitoza |
Hipofiza |
Kalcij |
Kalcij-vezalne beljakovine |
Hipokampus |
Hrbtenjača |
Mali možgani |
Mikroskopija konfokalna |
Podgane, Wistar |
Polimerazna, verižna reakcija |
RNA, prenašalna |
Snov-vnos, tehnike
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kreft, Marko | 15666 |
Dominko, Asja | 16342 |
Grilc, Sonja | 06846 |
Rupnik, Marjan | 12266 |
Milisav, Irina | 18825 |
Zorec, Robert, 1958- | 03702 |
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