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  • Molecular markers of Schwanniomyces (Debaryomyces) yeasts = Molekularni markerji Schwanniomyces (Debaryomyces) kvasovk
    Smole Možina, Sonja ; Rozman, Damjana, 1960- ; Raspor, Peter
    Two molecular genetic methods (karyotyping i.e. separation of intact chromosomes with pulsed field gel electrophoresis-CHEF PFGE and AP-PCR, i.e. PCR fingerprinting with randomly chosen ... oligonucleotide primers) have been tested for intra- and interspecific differentation of some Schwanniomyces and closely related yeast strains. Because of the large chromosomes (1000-2500 kb), which require prolonged electrophoretic separation protocols, AP-PCR fingerprinting revealed to be more effective for quick differentiation between Schwanniomyces yeast strains, but greater number of primers (deca-mer oligonucleotides with 50-80% G+C, microsatelite repeat sequences) should be tested for generation of strain specific and species specific RAPD markers. The result are discussed also in terms of classification sysstem of Schwanniomyces yeasts.
    Vir: Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo = Research reports [of the] Biotechnical Faculty University of Ljubljana. Agricultural issue. - ISSN 1408-340X (65, 1995, str. 73-80)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 1995
    Jezik - angleški
    COBISS.SI-ID - 1940856

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