-
Recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from plants, venoms and mites [Elektronski vir]Gattinger, Pia ...Background: N-linked glycans present in venoms, pollen and mites are recognized by IgE antibodies from >20% of allergic patients but have low or no allergenic activity. Objectives: To engineer ... recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from venoms, pollen and mites which can discriminate carbohydrate-specific IgE from allergenic, peptide-specific IgE. Methods: One or two N-glycosylation sites were engineered into the N-terminus of the non-allergenic protein horse heart myoglobin (HHM) using synthetic gene technology. HHM 1 and HHM 2 containing one or two N-glycosylation sites were expressed in baculovirus-infected High-FiveTM insect cells and a non-glycosylated version (HHM 0) was obtained by mutating the glycosylation motif. Recombinant HHM proteins were analyzed regarding fold and aggregation by circular dichroism and gel filtration, respectively. IgE reactivity was assessed by ELISA, immunoblotting and quantitative ImmunoCAP measurements. IgE inhibition assays were performed to study cross-reactivity with venom, plant and mite-derived carbohydrate IgE epitopes. Results: HHM-glycovariants were expressed and purified from insect cells as monomeric and folded proteins. The HHM-glycovariants exhibited strictly carbohydrate-specific IgE reactivity, designed to quantify carbohydrate specific IgE and resembled IgE epitopes of pollen, venom and mite-derived carbohydrates. IgE-reactivity and inhibition experiments established a hierarchy of plant glcyoallergens (nPhl p 4 > nCyn d 1 > nPla a 2 > nJug r 2 > nCup a 1 > nCry j 1) indicating a hitherto unknown heterogeneity of carbohydrate IgE epitopes in plants which were completely represented by HHM 2. Conclusion: Defined recombinant HHM-glycoproteins resembling carbohydrate-specific IgE epitopes from plants, venoms and mites were engineered which made it possible to discriminate carbohydrate- from peptide-specific IgE reactivity.Vir: EBioMedicine. - ISSN 2352-3964 (Vol. 39, Jan. 2019, str. 33-43)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2019Jezik - angleškiCOBISS.SI-ID - 2048486001
Avtor
Gattinger, Pia |
Mittermann, Irene |
Lupinek, Christian |
Hofer, Gerhard |
Keller, Walter, imunolog |
Bidovec, Urška |
Korošec, Peter, 1970- |
Koessler, Christine |
Novak, Natalija, zdravnica |
Valenta, Rudolf, 1963-
Teme
Allergy and immunology |
Allergens |
Glycoproteins |
Molecular diagnostic techniques |
Alergija in imunologija |
Alergeni |
Glikoproteini |
Molekularne diagnostične tehnike |
recombinant glycoproteins |
molecular allergology |
component-resolved diagnosis |
rekombinantni glikoproteini |
molekularna alergologija |
molekularna diagnostika
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Gattinger, Pia | |
Mittermann, Irene | |
Lupinek, Christian | |
Hofer, Gerhard | |
Keller, Walter, imunolog | |
Bidovec, Urška | 25169 |
Korošec, Peter, 1970- | 22807 |
Koessler, Christine | |
Novak, Natalija, zdravnica | |
Valenta, Rudolf, 1963- |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.