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Fakulteta za farmacijo, Ljubljana (FFALJ)
  • Udruženost polimorfizama Pro12Ala i His477His gena PPARG s inzulinskom rezistencijom u bolesnica sa sindromom policističnih jajnika = Association between Pro12Ala and His477His polymorphisms in PPARG gene and insulin resistance in patients with polycystic ovary syndrome
    Dragojevič, Jana ; Marc, Janja ; Mlinar, Barbara, 1974-
    Background: Insulin resistance is characterized by attenuated response of tissues to insulin action and is associated with obesity, type 2 diabetes mellitus, metabolic syndrome, lipodystrophies, ... polycystic ovary syndrome (PCOS) and chronic infection. Insulin resistance is present in 50%-70% of PCOSpatients. Peroxisome proliferator-activated receptor ? (PPAR?) is a nuclear receptor which controls transcription of genes involved in free fatty acid uptake and lipogenesis, and is essential for differentiation and survivalof adipocytes. The aim of our pilote study was to investigate the association between Pro12Ala and His477His polymorphisms in PPARG gene with insulin resistance in PCOS patients. Patients and methods: The study included 69 PCOS patients. Genotype frequencies were analysed by PCR-RFLP methods. Fasting glucose and glucose after oral glucose tolerance test, insulin, and high sensitivity C-reactive protein (hsCRP), body mass, height, waist circumference, systolic and diastolic blood pressure, body mass index (BMI) and homeostasis model assessment (HOMA) index were measured by routine methodsor calculated from the findings obtained. Results: A significant association was found between the Ala allele of the Pro12Ala polymorphism and lower BMI (P=0,040) and between the T allele of the His477His polymorphism andlower hsCRP (P=0,047). The Pro12Ala and the His477His polymorphisms were in considerable linkage disequilibrium (D' = 0,727). Diplotype analysis showedno association. Conclusions: In our preliminary study, we found a significant association between the Ala allele of the Pro12Ala polymorphism and lower BMI, and between the T allele of the His477His polymorphism and lower hsCRP. However, none of the two polymorphisms, individualy or in combination (diplotype), was associated with fasting plasma insulin and glucose concentrations and HOMA index that are the characteristics of insulin resistance. We therefore conclude that the polymorphisms investigated are not associated with insulin resistance in PCOS patients.
    Vir: Biochemia medica : glasnik Hrvatskoga društva medicinskih biokemičara. - ISSN 1330-0962 (vol. 18, no. 3, 2008, str. 342-350)
    Vrsta gradiva - članek, sestavni del
    Leto - 2008
    Jezik - angleški
    COBISS.SI-ID - 2496881

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