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Institut Jožef Stefan, Ljubljana (IJS)
  • Selenium species in leaves of chicory, dandelion, lamb's lettuce and parsley
    Mazej, Darja ; Osvald, Jože, 1943- ; Stibilj, Vekoslava
    LambḊs lettuce, dandelion, parsley and four cultivars of chicory were cultivated aeroponically for 41 days with nutrient solution containing 7 mg Se/L in the form of Na2SeO4. Se compounds were ... determined by high performance liquid chromatographyÉultraviolet treatmentÉhydride generation atomic fluorescence spectrometry (HPLCÉUVÉHG-AFS) in the green parts of the selected plants. Se species were extracted by water and by enzymatic hydrolysis with Protease XIV. Separation of SeIV, SeVI, SeMet, SeMeSeCys and SeCys2 was made by a combination of anion and cation exchange chromatography in which the columns were connected on-line to a UVÉHG-AFS detection system. Se accumulatedefficiently in plant leaves up to 480 ?g/g dry mass, mostly as SeVI, i.e. the form of Se in the nutrient solution. Beside inorganic Se, selenomethionine (6É21%), selenomethylselenocysteine (0.5É4.4%) and selenocistine (<DL-0.8%) were determined in the extracts after enzymatic hydrolysis. Some unidentified peaks were also observed in the chromatograms.
    Vir: Food chemistry. - ISSN 0308-8146 (Vol. 107, 2008, str. 75-83)
    Vrsta gradiva - članek, sestavni del
    Leto - 2008
    Jezik - angleški
    COBISS.SI-ID - 21229607

vir: Food chemistry. - ISSN 0308-8146 (Vol. 107, 2008, str. 75-83)

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