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  • The involvement of uric acid in the scavenging of nitroxide radicals by ascorbate
    Schara, Milan Valter ; Nemec, Marjanca ; Pečar, Slavko
    The reaction kinetics, measured by electron paramagnetic resonance (EPR) was used to study the involvement of uric acid in the reactions of nitroxide radical (TEMPONE) scavenging by ascorbate (PBS, ... pH 7.4 and T=37 C). In absence of iron ions, the oxygen reoxidation of hydroxylamine was slow and the addition of uric acid not change the observed kinetics. On the other hand, in presence of iron ions the reoxidation starts to recover the nitroxide radical. This reaction rate is strongly enhanced by uric acid, In fact there are two hypotheses, the first ascribes more efficient iron complexes with uric acid, by which the reoxidation increases. Especially the competition between the phosphate and uric acid ligands might be important. However, uric acid could be directly involved in scavenging the hydroxyl radicals, and herewith influence the reaction rate. No scavenging activity of uric acid in UV irradiated aqueous solutions was found. The trapped adducts of hydroxyl andthe superoxide radicals sho the same concetration in presence and absence of uric acid.
    Vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 47, no. 1, 2000, str. 39-46)
    Vrsta gradiva - članek, sestavni del
    Leto - 2000
    Jezik - angleški
    COBISS.SI-ID - 22021893

vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 47, no. 1, 2000, str. 39-46)

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