UNI-MB - logo
UMNIK - logo
 
Narodna in univerzitetna knjižnica, Ljubljana (NUK)
Naročanje gradiva za izposojo na dom
Naročanje gradiva za izposojo v čitalnice
Naročanje kopij člankov
Urnik dostave gradiva z oznako DS v signaturi
  • Matične celice površinskega epitelija jajčnika : oogeneza in vitro? = Ovarian surface epithelium stem cells : oogenesis in vitro?
    Virant-Klun, Irma ...
    The dogma that the total number of follicles and oocytes available for reproduction are determined at birth, and that one follicle and one oocyte arerecruited from the existing pool to mature in each ... menstrual cycle still persists. There is an increasing experimental evidence that this might not be true. In the ovarian surface epithelium there might be nondifferentiated stem cells that might differentiate into oocytes also in adult life. In this research we aimed at isolating putative stem cells from the ovarian surface epithelium and at evaluating in vitro oogenesis in the ovarian cell culture invitro in women without naturally present follicles and oocytes in their ovarian cortex - postmenopausal women and women with premature ovarian failure. Ovarian surface epithelium was scraped from the ovaries of 20 postmenopausal women and S women with premature ovarian failure. `e tried to find putative stem cells in ovarian scraping and to confirm them by transcription markers Oct-4, Sox-2, and Nanog, and by surface antigen SSEA-4. Cell culture was set up by scraped cells in DMEM/F-12 medium with phenol red, which shows a weak estrogenic activity. Ovarian cell culture was cultured for 20 days in a CO2 incubator at 3.7 degreesC and 5% COz. Development of cells inthe culture was followed and th epresence of oocyte-like cells was evaluatedby using different methods - evaluation of morphology, transcription markers, surface antigen markers, oocyte immunohistochemical markers, and flowcytometry after propidium iodide staining. (Abstract truncated at 2000 characters)
    Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 76, št. 10, okt. 2007, str. 603-613)
    Vrsta gradiva - članek, sestavni del
    Leto - 2007
    Jezik - slovenski
    COBISS.SI-ID - 23478745

    Povezava(-e):

    Digitalna knjižnica Slovenije - dLib.si

Vnos na polico