-
Bystander effect induced by electroporation is possibly mediated by microvesicles and dependent on pulse amplitude, repetition frequency and cell typePrevc, Ajda ...Bystander effect, a known phenomenon in radiation biology, where irradiated cells release signals which cause damage to nearby, unirradiated cells, has not been explored in electroporated cells yet. ... Therefore, our aim was to determine whether bystander effect is present in electroporated melanoma cells in vitro, by determining viability of non-electroporated cells exposed to medium from electroporated cells and by the release of microvesicles as potential indicators of the bystander effect. Here, we demonstrated that electroporation of cells induces bystander effect: Cells exposed to electric pulses mediated their damage to the non-electroporated cells, thus decreasing cell viability. We have shown that shedding microvesicles may be one of the ways used by the cells to mediate the death signals to the neighboring cells. The murine melanoma B16F1 cell line was found to be more electrosensitive and thus more prone to bystander effect than the canine melanoma CMeC-1 cell line. In B16F1 cell line, bystander effect was present above the level of electropermeabilization of the cells, with the threshold at 800 V/cm. Furthermore, with increasing electric field intensities and the number of pulses, the bystander effect also increased. In conclusion, electroporation can induce bystander effect which may be mediated by microvesicles, and depends on pulse amplitude, repetition frequency and cell type.Vir: The journal of membrane biology. - ISSN 0022-2631 (Vol. 249, iss. 5, Oct. 2016, str. 703-711)Vrsta gradiva - članek, sestavni delLeto - 2016Jezik - angleškiCOBISS.SI-ID - 2389627
Avtor
Prevc, Ajda |
Bedina Zavec, Apolonija |
Čemažar, Maja |
Kloboves-Prevodnik, Veronika |
Savarin, Monika |
Todorović, Vesna, 1982- |
Strojan, Primož |
Serša, Gregor
Teme
elektroporacija |
mikromehurčki |
melanomske celice |
ionizirajoče sevanje |
bystander effect |
ionizing radiation |
electroporation |
microvesicles |
melanoma cell lines
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Prevc, Ajda | 37415 |
Bedina Zavec, Apolonija | 16104 |
Čemažar, Maja | 14575 |
Kloboves-Prevodnik, Veronika | 15076 |
Savarin, Monika | 35355 |
Todorović, Vesna, 1982- | 29469 |
Strojan, Primož | 14576 |
Serša, Gregor | 08800 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.