We report the first documented case of OXA-48-producing Klebsiella pneumoniae in Slovenia isolated from rectal surveillance cultures from a patient transferred from Libya. The patient was colonised ...with both ESBL-producing Escherichia coli and ESBL- and OXA-48-producing K. pneumoniae. Three further patients were colonised with ESBL-producing E. coli. This underscores the importance of an early warning system on European level and screening upon admission of patients transferred across borders and between healthcare systems.
We studied the occurrence of the parasite Blastocystis hominis in 1066 stool specimens from patients with diarrhoea, and investigated the relationship between the presence of B. hominis in the faeces ...and the age of patients. The parasite was recovered from 3.7% samples, but as the sole species of micro-organism in the stool it was recovered from 1% samples. There was no statistically significant difference in the number of B. hominis-positive stools between the younger and the older patients (P < 0.25), yet in the latter, B. hominis was more frequently identified as the only species of micro-organism as compared with the younger group (P < 0.005). The presence of B. hominis in faecal samples of patients with diarrhoea harbouring no other intestinal pathogens suggests an aetiology that should receive more attention in Slovenia.
Organisms referred to as cyanobacterium-like or coccidian-like bodies (CLB) are now thought to belong to the coccidian genus Cyclospora. For this newly described organism the name Cyclospora ...cayetanensis has been proposed. Since several outbreaks of diarrhoea caused by C. cayetanensis have been reported worldwide during the past few years, and as no such data are yet available for Slovenia, we decided to evaluate the occurrence of this protozoon as a pathogenic agent in patients with diarrhoea in this country.
DNA‐encoded combinatorial synthesis provides efficient and dense coverage of chemical space around privileged molecular structures. The indole side chain of tryptophan plays a prominent role in key, ...or “hot spot”, regions of protein–protein interactions. A DNA‐encoded combinatorial peptoid library was designed based on the Ugi four‐component reaction by employing tryptophan‐mimetic indole side chains to probe the surface of target proteins. Several peptoids were synthesized on a chemically stable hexathymidine adapter oligonucleotide “hexT”, encoded by DNA sequences, and substituted by azide‐alkyne cycloaddition to yield a library of 8112 molecules. Selection experiments for the tumor‐relevant proteins MDM2 and TEAD4 yielded MDM2 binders and a novel class of TEAD‐YAP interaction inhibitors that perturbed the expression of a gene under the control of these Hippo pathway effectors.
A focused approach: A DNA‐encoded peptoid library was designed by the Ugi multicomponent reaction around indole structures that mimic the side chain of tryptophan. Applying this focused library to the challenging cancer targets MDM2 and hTEAD4 yielded compounds for inhibitor development. Compounds binding to hTEAD4 disrupted the hTEAD4–YAP interaction, and reduced expression of a gene under control of the TEAD–YAP transcription factor complex.
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