Stevia rebaudiana (Bertoni) is known as a natural sweetener plant to produced steviol glycosides. The steviol glycosides biosynthesis is limited in S. rebaudiana plants due to the alteration in the ...environmental circumstances such as drought, cold, salt and light. These environmental circumstances are a common side-effect in plants affecting the plant growth, metabolism and yield of secondary metabolites. Due to absence of complete genome annotations, the plant metabolites signaling is difficult in order to get the exact enzymatic flow to the product. In this article, we have analyzed the ESTs of S. rebaudiana and predicted their role in plant signaling in term of cis-regulatory elements, their biological function respect to abiotic stress. Further, the predicted abiotic stress responsive factors were also analyzed in order to predict the relevant genes or proteins function in comparison with the genome of Arabidopsis thaliana. Total 5,548 ESTs of stevia were retrieved from NCBI database. EST-contigs assembled from 5393 were 619 contigs and 2,894 singletons elements were identified by assembler program. Due to short expressed sequences related to singletons, it is excluded for further study. Further, retrieved ESTs were resulted in to 619 EST-contigs by using the clustering method. Out of 619, 15 contigs belongs to transcription factor families while 292 contigs, belongs to five enzyme classes. Out of 619 contigs, the 529 contigs showing the correct gene ontology in term of biological process (BP), molecular Functions (MF) and cellular component (CC). Further, these contigs were also screened for metabolic pathways analyses using KEG database. In this, 390 metabolic pathways and 67 involved for signal transduction were identified from 619 contigs. The co-expression analysis was revealed by gene investigators and STRING 10.0 with 0.40 correlations and 0.9 mutual connection. In projected PPI network, the recognized factors (WD40-like protein, MYB-HB like, AP2-EREBP, C2H2, Hap3/NF-YB, bHLH, C2C2-CO-like, CW-Zn, FHA-SMAD, Nin like, SBP3, TIFY, Tc-PD, Znf-B and bIP) belong to plant signaling and MAPK signaling pathways. These TFs introduce as a candidate genes responsive factors may lead to enhanced plant growth and metabolism by overexpression.
Pot experiments were conducted to investigate the role of selenium in alleviating cadmium stress in
Solanum lycopersicum
seedlings. Cadmium (150 mg L
−1
) treatment caused a significant reduction in ...growth in terms of height and biomass accumulation and affected chlorophyll pigments, gas exchange parameters, and chlorophyll fluorescence. Selenium (10 μM) application mitigated the adverse effects of cadmium on growth, chlorophyll and carotenoid contents, leaf relative water content, and other physiological attributes. Lipid peroxidation and electrolyte leakage increased because of cadmium treatment and selenium-treated plants exhibited considerable reduction because of the decreased production of hydrogen peroxide in them. Cadmium-treated plants exhibited enhanced activity of antioxidant enzymes that protected cellular structures by neutralizing reactive free radicals. Supplementation of selenium to cadmium-treated plants (Cd + Se) further enhanced the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) by 19.69, 31.68, 33.14, and 54.47%, respectively. Osmolytes, including proline and glycine betaine, increased with selenium application, illustrating their role in improving the osmotic stability of
S. lycopersicum
under cadmium stress. More importantly, selenium application significantly reduced cadmium uptake. From these results, it is clear that application of selenium alleviates the negative effects of cadmium stress in
S. lycopersicum
through the modifications of osmolytes and antioxidant enzymes.
This study assessed the effects of 24-epibrassinolide (EBL, 10
M) and silicon (2 mM) on the alleviation of cadmium (Cd, 150 mg L
) toxicity in Pisum sativum L. seedlings via the modulation of growth, ...antioxidant defense, glyoxalase system, and nutrient uptake.
Shoot and root lengths declined by 46.43% and 52.78%, respectively, following Cd stress. Shoot and root dry weights also declined with Cd toxicity. Biochemical and physiological aspects exhibit significant decline including total chlorophyll (33.09%), carotenoid (51.51%), photosynthetic efficiency (32.60%), photochemical quenching (19.04%), leaf relative water content (40.18%), and gas exchange parameters (80.65%). However, EBL or Si supplementation alone or in combination modulates the previously mentioned parameters. Cadmium stress increased proline and glycine betaine (GB) contents by 4.37 and 2.41-fold, respectively. Exposure of plants to Cd stress increased the accumulation of H
O
, malondialdehyde content, electrolyte leakage, and methylglyoxal, which declined significantly with EBL and Si supplementation, both individually and in combination. Similarly, Cd stress adversely affected enzymatic and non-enzymatic antioxidants, but EBL and/or Si supplementation maintained antioxidant levels. Glyoxalase I (GlyI) accumulated after Cd stress and increased further with the application of EBL and Si. However, GlyII content declined after Cd stress but increased with supplementation of EBL and Si. Cadmium accumulation occurred in the following order: roots > shoots>leaves. Supplementation with EBL and Si, individually and in combination reduced Cd accumulation and enhanced the uptake of macronutrients and micronutrients in shoots and roots, which declined with Cd toxicity.
The application of 24-EBL and Si, individually and in combination, alleviated the adverse effects of Cd by improving growth, biochemical parameters, nutrient uptake, osmolyte accumulation, and the anti-oxidative defense and glyoxalase systems in Pisum sativum seedlings.
Environmental pollution by alkaline salts, such as Na
CO
, is a permanent problem in agriculture. Here, we examined the putative role of jasmonic acid (JA) in improving Na
CO
-stress tolerance in ...maize seedlings. Pretreatment of maize seedlings with JA was found to significantly mitigate the toxic effects of excessive Na
CO
on photosynthesis- and plant growth-related parameters. The JA-induced improved tolerance could be attributed to decreased Na uptake and Na
CO
-induced oxidative damage by lowering the accumulation of reactive oxygen species and malondialdehyde. JA counteracted the salt-induced increase in proline and glutathione content, and significantly improved ascorbic acid content and redox status. The major antioxidant enzyme activities were largely stimulated by JA pretreatment in maize plants exposed to excessive alkaline salts. Additionally, increased activities of glyoxalases I and II were correlated with reduced levels of methylglyoxal in JA-pretreated alkaline-stressed maize plants. These results indicated that modifying the endogenous Na
and K
contents by JA pretreatment improved alkaline tolerance in maize plants by inhibiting Na uptake and regulating the antioxidant and glyoxalase systems, thereby demonstrating the important role of JA in mitigating heavy metal toxicity. Our findings may be useful in the development of alkali stress tolerant crops by genetic engineering of JA biosynthesis.
The protective role of exogenously applied kinetin (10 μM KN, a cytokinin) against the adverse effects caused by NaCl-induced (150 mM) stress in Solanum lycopersicum was investigated. Application of ...KN significantly enhanced growth and biomass production of normally grown plants (non-stressed) and also mitigated the adverse effect of NaCl on stressed plants to a considerable extent. Among the examined parameters, chlorophyll and carotenoid contents, photosynthetic parameters, components of the antioxidant system (both enzymatic and non-enzymatic), osmotica accumulation, and mineral uptake exhibited a significant increase following the application of KN. Furthermore, KN application reduced the generation of reactive free radical hydrogen peroxide, coupled with a significant reduction in lipid peroxidation and an increase in membrane stability. The activities of antioxidant enzymes, and glyoxylase system were found to be promoted in plants exposed to NaCl, and the activities were further promoted by KN application, thereby protecting S. lycopersicum plants against NaCl-induced oxidative damage. Further strengthening of the antioxidant system in KN supplied plants was ascribed to regulation of ascorbate-glutathione cycle, phenols and flavonoids in them. The levels of proline and glycine betaine increased considerably in KN-treated plants, thereby maintaining relative water content. Moreover, exogenous KN application reduced the inhibitory effects of NaCl on K+ and Ca2+ uptake, which resulted in a considerable reduction in tissue Na+/K+ ratio.
Reactive oxygen species (ROS, partial reduction or derivatives of free radicals) are highly reactive, dangerous and can cause oxidative cell death. In addition to their role as toxic by-products of ...aerobic metabolism, ROS play a role in the control and regulation of biological processes such as growth, the cell cycle, programmed cell death, hormone signaling, biotic and abiotic stress reactions and development. ROS always arise in plants as a by-product of several metabolic processes that are located in different cell compartments, or as a result of the inevitable escape of electrons to oxygen from the electron transport activities of chloroplasts, mitochondria and plasma membranes. These reactive species are formed in chloroplasts, mitochondria, plasma membranes, peroxisomes, apoplasts, the endoplasmic reticulum and cell walls. The action of many non-enzymatic and enzymatic antioxidants present in tissues is required for efficient scavenging of ROS generated during various environmental stressors. The current review provides an in-depth look at the fate of ROS in plants, a beneficial role in managing stress and other irregularities. The production sites are also explained with their negative effects. In addition, the biochemical properties and sources of ROS generation, capture systems, the influence of ROS on cell biochemistry and the crosstalk of ROS with other signaling molecules/pathways are discussed.
Recently, the application of salicylic acid (SA) for improving a plant's resistance to abiotic stresses has increased. A large part of the irrigated land (2.1% out of 19.5%) is severely affected by ...salinity stress worldwide. In 2020, total production of wheat (
) was 761 million tons, representing the second most produced cereal after maize; therefore, research on its salinity tolerance is of world concern. Photosynthetic attributes such as net photosynthetic rate (P
), stomatal conductance (gs), intercellular CO
concentration (Ci), and transpiration rate (E) were increased significantly by the application of SA. Salt stress increased antioxidant enzyme activity; however, SA further boosted their activity along with proline level. We conclude that SA interacts with meristematic cells, thereby triggering biochemical pathways conductive to the increment in morphological parameters. Further research is required to dissect the mechanisms of SA within the wheat plants under stress.
Cucumber is an essential vegetable crop throughout the world. Cucumber development is vital for accomplishing both quality and productivity requirements. Meanwhile, numerous factors have resulted in ...substantial cucumber losses. However, the calreticulin domain-encoding genes (CDEGs) in cucumber were not well-characterized and had little function. In the genome-wide association study (GWAS), we recognized and characterized the CDEGs in Cucumis sativus (cucumber). Through a comprehensive study of C. sativus, our research has unveiled the presence of three unique genes, denoted as CsCRTb, CsCRT3, and CsCNX1, unevenly distributed on three chromosomes in the genome of C. sativus. In accordance to the phylogenetic investigation, these genes may be categorized into three subfamilies. Based on the resemblance with AtCDE genes, we reorganized the all CsCDE genes in accordance with international nomenclature. The expression analysis and cis-acting components revealed that each of CsCDE gene promoter region enclosed number of cis-elements connected with hormone and stress response. According to subcellular localization studies demonstrated that, they were found in deferent locations of the cell such as endoplasmic reticulum, plasma membrane, golgi apparatus, and vacuole, according to subcellular localization studies. Chromosomal distribution analysis and synteny analysis demonstrated the probability of segmental or tandem duplications within the cucumber CDEG gene family. Additionally, miRNAs displayed diverse modes of action, including mRNA cleavage and translational inhibition. We used the RNA seq data to analyze the expression of CDEG genes in response to cold stress and also improved cold tolerance, which was brought on by treating cucumber plants to an exogenous chitosan oligosaccharide spray. Our investigation revealed that these genes responded to this stress in a variety of ways, demonstrating that they may adapt quickly to environmental changes in cucumber plants. This study provides a base for further understanding in reference to CDE gene family and reveals that genes play significant functions in cucumber stress responses.
The salt stress limits the production of mustard throughout the world and it is one of the major abiotic stresses. Crop productivity is declining due to the limited area of fertile land. In order to ...investigate the effects NO donor on salt tolerance and the recovery of
Brassica juncea.
(L) cv. RGN-48, sodium nitroprusside (SNP) was applied at 1, 10 or 100 µM concentrations as foliar spray for five days consecutively. SNP triggered a significant increase in the main antioxidative enzymes including catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD) along with the increase in the enzymes involved in nitrogen metabolism (nitrate reductase), photosynthesis and respiration (carbonic anhydrase, rubisco, fumarase, hexokinase and succinate dehydrogenase). On the other hand, decrease in programmed cell death (PCD) and the contents of hydrogen peroxide (H
2
O
2
), superoxide anion (O
2
.−
) and malondialdehyde (MDA) was observed in NaCl-stressed plants subjected to the different concentrations of SNP. Consequently, the spray of SNP restored several photosynthetic attributes i.e. SPAD chlorophyll, chlorophyll fluorescence and gas exchange parameters in NaCl-stressed plants. These results suggested that exogenous application of SNP is useful in ameliorating the toxicity generated by NaCl in mustard plants.
In the last few decades, use of copper oxide nanoparticles (CuO NPs) has been increased significantly that eventually included as a growth stimulator. This makes it essential to examine their impact ...on several plants. In the study detailed here, we investigated the effects of CuO NPs on the growth, physiological efficiency, biochemical assays, and antioxidant system in the mustard plant. Varying concentrations (0, 2, 4, 8, and 16 mg/L) of CuO NPs were applied at 25 days after sowing (DAS), and sampling took place at 30 and 45 DAS. The results indicate that CuO NPs-treated plants registered an increase in the growth and biomass over their respective control. Among different concentrations of CuO NPs (0, 2, 4, 8, and 16 mg/L), 8 mg/L proved to be the optimum foliar spray treatment and increase the chlorophyll content, net photosynthetic rate, leaf proline content, and antioxidant enzymes activity. We concluded that CuO NPs interact with meristematic cells triggering biochemical pathways conductive to an enhancement of the growth attributes. Further studies are needed to investigate the mechanisms of CuO NPs in mustard.
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