Elemental analysis of cellular samples by in-air micro-PIXE Ishii, K; Sugimoto, A; Tanaka, A ...
Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms,
07/2001, Letnik:
181, Številka:
1
Journal Article
Recenzirano
We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in ...a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.
We developed a novel technique to obtain 3D-imaging of elemental distribution based on multi-angle RBS measurement and micro-beam scanning. Since cross-sections of RBS are too small to obtain ...3D-imaging, it is necessary to measure scattered particles with large solid angle detectors. Hence, the RBS profiles from each scattering angle are superposed and a true depth profile cannot be obtained. Recently, a large solid angle X-ray detector array consisting of 45 detector elements was installed at the TIARA facility of Japan Energy Research Institute. Each detector element measures X-rays as well as scattered particles. For this utilization, we introduced a technique to superpose RBS profiles and applied it to the surface analysis of an ant’s head.
The pre-amplifier with an active reset system was examined to carry out PIXE analysis under the condition that X-rays and back scattered protons are detected simultaneously. Rejection of false ...signals produced by back scattered protons is discussed, and it is confirmed that false signals can be completely rejected by using an inhibit signal of the preamplifier. Energy resolution of an X-ray detector and a live-time of measurement system were measured as a function of counting rate. As a result, energy resolution did not change and the live-time was 70 % at the high counting rate over 3kcps. The pile-up effect on the X-ray signal with the proton signal tail is also discussed.
