For years, an increasing number and diversity of genetically modified plants has been grown on a commercial scale. The need for detection and identification of these genetically modified organisms ...(GMOs) calls for broad and at the same time flexible high throughput testing methods. Here we describe the development and validation of a hexaplex real-time polymerase chain reaction (PCR) screening assay covering more than 100 approved GMOs containing at least one of the GMO targets of the assay. The assay comprises detection systems for Cauliflower Mosaic Virus 35S promoter, Agrobacterium tumefaciens NOS terminator, Figwort Mosaic Virus 34S promoter and two construct-specific sequences present in novel genetically modified soybean and maize that lack common screening elements. Additionally a detection system for an internal positive control (IPC) indicating the presence or absence of PCR inhibiting substances was included. The six real-time PCR systems were allocated to five detection channels showing no significant crosstalk between the detection channels. As part of an extensive validation, a limit of detection (LODabs) ≤ ten target copies was proven in hexaplex format. A sensitivity ≤ ten target copies of each GMO detection system was still shown in highly asymmetric target situations in the presence of 1,000 copies of all other GMO targets of each detection channel. Furthermore, the applicability to a broad sample spectrum and reliable indication of inhibition by the IPC system was demonstrated. The presented hexaplex assay offers sensitive and reliable detection of GMOs in processed and unprocessed food, feed and seed samples with high efficiency. graphic removed
Abstract
Building on the innovative design of the In-Vacuum APPLE IVUE32, a design for a 15mm period cryogenic elliptical undulator (CPMUE15) is proposed. The undulator is to be developed under the ...ATHENA collaboration. Initially designed to provide a radiator for the SINBAD facility at DESY, a comparison is made for a design to provide an Afterburner device for FLASH1.
Abstract
At BESSY II, two new beamlines for RIXS and for X-Ray-microscopy need a short period variably polarizing undulator. For this purpose, the first in-vacuum APPLE II undulator worldwide is ...under construction. The parameters are as follows: period length
λ
0
= 32
mm, # periods
= 78,
minimum gap = 7mm
. The design incorporates a force compensation scheme as proposed by two of the authors at the SRI 2018. All precision parts of the drive chain are located in air. New transverse slides for the transversal slit adjustment have been developed and tested. Optical Micrometers measure the gap and phase positions, similar to the system of the CPMU17 at BESSY II. They provide the signals for motor feedback loops. A new UHV-compatible soldering technique, as developed with industry, relaxes fabrication tolerances of magnets and magnet holders and simplifies the magnet assembly. A 10-period prototype has been setup for lifetime tests of the new magnetic keeper design. The paper summarizes the status of the undulator IVUE32.
The orientation of magnetic moments at the (100) surface of antiferromagnetic NiO single crystals is studied by x-ray linear magnetic dichroism in photoemission microscopy. T domains are observed ...terminating at the surface, with domain boundaries running mostly along in-plane 10 directions. From the detailed polarization dependence we find that the magnetic surface structure of a cleaved crystal is bulk terminated. This is in contrast to sputtered surfaces, where magnetic moments lie within the surface plane, forming a magnetically relaxed structure. These findings are of importance for understanding the exchange bias phenomenon.
We have demonstrated the generation of circularly polarized coherent light pulses at 66 nm wavelength by combining laser seeding at 263 nm of a 375 MeV relativistic electron bunch with subsequent ...coherent harmonic generation from an elliptical undulator of APPLE-II type. Coherent pulses at higher harmonics in linear polarization have been produced and recorded up to the sixth order (44 nm). The duration of the generated pulses depends on the temporal overlap of the initial seed laser pulse and the electron bunch and was on the order of 200 fs. Currently, this setup is the only source worldwide producing coherent fs-light pulses with variable polarization in the vacuum ultraviolet.
The elliptically polarized undulator beamlines at BESSY II Weiss, M.R.; Follath, R.; Sawhney, K.J.S. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
07/2001, Letnik:
467, Številka:
1
Journal Article
Recenzirano
During 1998/99 three beamlines of the UE56-PGM type were built at BESSY-II to meet the increased need for synchrotron radiation of tuneable, i.e. linear to fully circular, polarization. We report on ...the results of characterization measurements, which were focused on spectral resolution, photon flux and polarization properties.
The spectral resolution for all three beamlines exceeds
E/Δ
E=100
000 at 64.1
eV as obtained from absorption spectra of doubly excited He. The flux measurements resulted in values that closely follow the theoretical prediction of up to 1×10
14
photons/(s
100 mA 0.1%) at 160
eV. The degree of polarization turned out to be close to 100% with circular and linear content, each of up to 100% depending on the undulator shift value, as predicted by theory. We also give first results on the double beam mode in which both undulator modules produce horizontally separated beams. This mode was used for fast switching of the polarization, utilizing a motorized chopper in the beamline.
This paper presents first results concerning the three-dimensional ultrasonic data acquisition, the textural analysis of different classes of tissues and the tissue-specific display of mastopathic ...regions within the female breast, revealing its benefit for diagnosis.
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