Mitochondrial DNA (mtDNA) copy number in peripheral blood is associated with increased risk of several cancers. However, data from prospective studies on mtDNA copy number and breast cancer risk are ...lacking. We evaluated the association between mtDNA copy number in peripheral blood and breast cancer risk in a nested case-control study of 183 breast cancer cases with pre-diagnostic blood samples and 529 individually matched controls among participants of the Singapore Chinese Health Study. The mtDNA copy number was measured using real time PCR. Conditional logistic regression analyses showed that there was an overall positive association between mtDNA copy number and breast cancer risk (P(trend) = 0.01). The elevated risk for higher mtDNA copy numbers was primarily seen for women with <3 years between blood draw and cancer diagnosis; ORs (95% CIs) for 2(nd), 3(rd), 4(th), and 5(th) quintile of mtDNA copy number were 1.52 (0.61, 3.82), 2.52 (1.03, 6.12), 3.12 (1.31, 7.43), and 3.06 (1.25, 7.47), respectively, compared with the 1(st) quintile (P(trend) = 0.004). There was no association between mtDNA copy number and breast cancer risk among women who donated a blood sample ≥3 years before breast cancer diagnosis (P(trend) = 0.41). This study supports a prospective association between increased mtDNA copy number and breast cancer risk that is dependent on the time interval between blood collection and breast cancer diagnosis. Future studies are warranted to confirm these findings and to elucidate the biological role of mtDNA copy number in breast cancer risk.
Social dilemmas are central to human society. Depletion of natural resources, climate protection, security of energy supply, and workplace collaborations are all examples of social dilemmas. Since ...cooperative behaviour in a social dilemma is individually costly, Nash equilibrium predicts that humans should not cooperate. Yet experimental studies show that people do cooperate even in anonymous one-shot interactions. In spite of the large number of participants in many modern social dilemmas, little is known about the effect of group size on cooperation. Does larger group size favour or prevent cooperation? We address this problem both experimentally and theoretically. Experimentally, we find that there is no general answer: it depends on the strategic situation. Specifically, we find that larger groups are more cooperative in the Public Goods game, but less cooperative in the N-person Prisoner's dilemma. Theoretically, we show that this behaviour is not consistent with either the Fehr &Schmidt model or (a one-parameter version of) the Charness &Rabin model, but it is consistent with the cooperative equilibrium model introduced by the second author.
We assessed the feasibility of a highly sensitive immunoassay method based on single molecule array (Simoa) technology to detect IgG and IgA antibodies against SARS-CoV-2 spike protein receptor ...binding domain (RBD) in saliva from individuals with natural or vaccine-induced COVID-19 immunity. The performance of the method was compared to a laboratory-developed SARS-CoV-2 RBD total antibody enzyme-linked immunosorbent assay (ELISA). Paired serum and saliva specimens were collected from individuals (n = 40) prior to and 2 weeks after receiving an initial prime COVID-19 vaccine dose (Pfizer/BioNTech BNT162b2 or Moderna mRNA-1273). Saliva was collected using a commercially available collection device (OraSure Inc.) and SARS-CoV-2 RBD IgG antibodies were measured by an indirect ELISA using concentrated saliva samples and a Simoa immunoassay using unconcentrated saliva samples. The IgG results were compared with paired serum specimens that were analyzed for total RBD antibodies using the ELISA method. The analytical sensitivity of the saliva-based Simoa immunoassay was five orders of magnitude higher than the ELISA assay: 0.24 pg/mL compared to 15 ng/mL. The diagnostic sensitivity of the saliva ELISA method was 90% (95% CI 76.3-97.2%) compared to 91.7% (95% CI 77.5-98.2%) for the Simoa immunoassay without total IgG-normalization and 100% (95% CI 90.3-100%) for the Simoa immunoassay after total IgG-normalization when compared to the serum ELISA assay. When analyzed using the SARS-CoV-2 RBD IgG antibody ELISA, the average relative increase in antibody index (AI) between the saliva of the post- and pre-vaccinated individuals was 8.7 (AI
). An average relative increase of 431 pg/mL was observed when the unconcentrated saliva specimens were analyzed using the Simoa immunoassay (SARS-CoV-2 RBD IgG
). These findings support the suitability of concentrated saliva specimens for the measurement of SARS-CoV-2 RBD IgG antibodies via ELISA, and unconcentrated saliva specimens for the measurement of SARS-CoV-2 RBD IgG and IgA using an ultrasensitive Simoa immunoassay.
Finding messaging to promote the use of face masks is fundamental during a pandemic. Study 1 (N = 399) shows that telling people to “rely on their reasoning” increases intentions to wear a face mask, ...compared with telling them to “rely on their emotions.” In Study 2 (N = 591) we add a baseline. However, the results show only a non‐significant trend. Study 3 reports a well‐powered replication of Study 2 (N = 930). In line with Study 1, this study shows that telling people to “rely on their reasoning” increases intentions to wear a face mask, compared to telling them to “rely on their emotions.” Two internal meta‐analyses show that telling people to “rely on their reasoning” increases intentions to wear a face mask compared (1) to telling them to “rely on their emotions” and (2) to the baseline. These findings suggest interventions to promote intentions to wear a face mask.
In a world in which many pressing global issues require large scale cooperation, understanding the group size effect on cooperative behavior is a topic of central importance. Yet, the nature of this ...effect remains largely unknown, with lab experiments insisting that it is either positive or negative or null, and field experiments suggesting that it is instead curvilinear. Here we shed light on this apparent contradiction by considering a novel class of public goods games inspired to the realistic scenario in which the natural output limits of the public good imply that the benefit of cooperation increases fast for early contributions and then decelerates. We report on a large lab experiment providing evidence that, in this case, group size has a curvilinear effect on cooperation, according to which intermediate-size groups cooperate more than smaller groups and more than larger groups. In doing so, our findings help fill the gap between lab experiments and field experiments and suggest concrete ways to promote large scale cooperation among people.
Are humans intuitively altruistic, or does altruism require self-control? A theory of social heuristics, whereby intuitive responses favor typically successful behaviors, suggests that the answer may ...depend on who you are. In particular, evidence suggests that women are expected to behave altruistically, and are punished for failing to be altruistic, to a much greater extent than men. Thus, women (but not men) may internalize altruism as their intuitive response. Indeed, a meta-analysis of 13 new experiments and 9 experiments from other groups found that promoting intuition relative to deliberation increased giving in a Dictator Game among women, but not among men (Study 1, N = 4,366). Furthermore, this effect was shown to be moderated by explicit sex role identification (Study 2, N = 1,831): the more women described themselves using traditionally masculine attributes (e.g., dominance, independence) relative to traditionally feminine attributes (e.g., warmth, tenderness), the more deliberation reduced their altruism. Our findings shed light on the connection between gender and altruism, and highlight the importance of social heuristics in human prosociality.
Toward defining commutative cubes in all dimensions, Brown and Spencer introduced the notion of “connection” as a new kind of degeneracy. In this paper, for a cubical set with connections, we show ...that the connections generate an acyclic subcomplex of the chain complex of the cubical set. In particular, our results show that the homology groups of a cubical set with connections are independent of whether we normalize by the connections or we do not, that is, connections do not contribute to any nontrivial cycle in the homology groups of the cubical set.
Variability induced by delayed cell processing and cell cryopreservation presents unique challenges for immunophenotyping in large population studies. We conducted a pilot study to evaluate the ...effect of delayed cell processing and cryopreservation on cell percentages obtained by immunophenotyping. We collected blood from 20 volunteers and compared the effect of (a) delayed cell processing up to 72 h (b) cryopreservation and (c) the combined effect of delayed cell processing and cryopreservation on immunophenotyping of 31 cell subsets that included several subsets of T, B, Natural Killer (NK) cells, monocytes and dendritic cells using both whole blood collected in EDTA tubes and peripheral blood mononuclear cells collected in CPT tubes. We found the delayed cell processing up to 72 h or cryopreservation alone did not significantly affect the percentages T cells, dendritic cells or monocytes but significantly increased the percentage of B cells and NK cells (p for trend ≤0.01) but. However combination of delayed cell processing up to 72 h and cryopreservation significantly increased the percentage of T cells as compared to cells processed immediately (p for trend <0.0001) while a delayed cell processing followed by cryopreservation decreased the percentage of NK cells (p for trend <0.0001). Total B-cells increased significantly with a 24–48 h delay in cell processing and cryopreservation but not at 72 h. The percentages of monocytes and dendritic cells remained unaffected by the combination of delayed cell processing and cryopreservation. These findings suggest that immunophenotyping of several immune cell subsets can be successfully implemented in large population studies as long as blood is processed within 48 h of biospecimen collection though some cell subsets may be more susceptible to a combination of delayed cell processing and cryopreservation.
Discrete homology theory for metric spaces Barcelo, Hélène; Capraro, Valerio; White, Jacob A.
Bulletin of the London Mathematical Society,
October 2014, 2014-10-00, Letnik:
46, Številka:
5
Journal Article
Recenzirano
Odprti dostop
We define and study a notion of discrete homology theory for metric spaces. Instead of working with simplicial homology, our chain complexes are given by Lipschitz maps from an n‐dimensional cube to ...a fixed metric space. We prove that the resulting homology theory satisfies a discrete analogue of the Eilenberg–Steenrod axioms, and prove a discrete analogue of the Mayer–Vietoris exact sequence. Moreover, this discrete homology theory is related to the discrete homotopy theory of a metric space through a discrete analogue of the Hurewicz theorem. We study the class of groups that can arise as discrete homology groups and, in this setting, we prove that the fundamental group of a smooth, connected, metrizable, compact manifold is isomorphic to the discrete fundamental group of a ‘fine enough’ rectangulation of the manifold. Finally, we show that this discrete homology theory can be coarsened, leading to a new non‐trivial coarse invariant of a metric space.
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