Wound healing effect of TENS in rodents Barreto, André Sales; de Souza Siqueira Barreto, Rosana; de Souza Siqueira Quintans, Jullyana ...
The FASEB journal,
04/2013, Letnik:
27, Številka:
S1
Journal Article
Recenzirano
Abstract only
The aim of this study was to investigate the wound healing activity of low frequency and high frequency TENS on excision wound model in rats.
Excision wound:
Thirty rats,
Wistar
, ...250–300 g were anesthetized and an excisional wound was performed. The animals was treated with placebo ‐ TENS simulation (equipment off), low‐frequency TENS (2 Hz) and high‐frequency TENS (100 Hz) for 7 days. The residual wounds were analyzed and removed. Experimental protocols were approved by the animal care and use committee at Federal University of Sergipe (CEPA 69/2011). Serial histological sections were obtained and stained by histochemical techniques (Hematoxilin‐Eosin – HE and Sirius red) and immunohistochemical techniques. The results suggest that the high‐frequency TENS reduced (p < 0.05) wound areas. This evidence shows that high‐frequency TENS modulates the wound healing process.
O consumo da dieta ocidental está associado ao surgimento de doenças cardiovasculares. O treinamento de resistência (TR) tem sido utilizado no tratamento destas doenças. O objetivo deste trabalho foi ...avaliar o efeito do TR sobre as alterações cardiometabólicas na prole de ratas expostas a dieta ocidental no período perinatal.
Ratas Wistar receberam dieta controle ou ocidental durante a gravidez e lactação. Os filhotes foram divididos em três grupos: Controle (C), dieta ocidental sedentário (OCS) e dieta ocidental+TR (OCTR). Aos 60 dias de vida, os animais iniciaram o protocolo de TR realizado cinco vezes por semana durante quatro semanas. Ao fim, foi registrada pressão arterial para análise da variabilidade da frequência cardíaca e sensibilidade do barorreflexo (SBR). Amostras de sangue foram coletadas para análise bioquímica.
O TR foi capaz de reduzir a pressão arterial, a modulação simpática vascular e aumentar a SBR. Houve melhoria no perfil bioquímico, com redução na glicemia de jejum, colesterol total e lipoproteínas de baixa densidade, além de aumento das lipoproteínas de alta densidade.
O TR promoveu adaptações benéficas ao sistema cardiovascular, mediadas por ajustes nos mecanismos de controle autonômico e perfil bioquímico dos animais expostos à dieta ocidental no período perinatal.
Consumption of a Western diet during the perinatal period is associated with development of cardiovascular disease. Resistance training (RT) has been used to treat cardiovascular disorders. The aim of this work was to assess the effect of RT on cardiometabolic disorders in rats exposed to a Western diet in the perinatal period.
Female Wistar rats were fed with control or Western diet during pregnancy and lactation. The pups were divided into three groups: Control (C), Western Diet Sedentary (WDS) and Western Diet + RT (WDRT). At 60 days of age, all animals started the RT protocol (five times a week for four weeks). At the end, blood pressure was recorded for analysis of heart rate variability and baroreflex sensitivity (BRS). Blood samples were collected for biochemical analysis.
RT reduced blood pressure and vascular sympathetic modulation and increased BRS. There were improvements in biochemical profile, with reductions in fasting blood glucose, total cholesterol and low‐density lipoprotein, and an increase in high‐density lipoprotein.
RT led to beneficial adaptations in the cardiovascular system, mediated by changes in the mechanisms of autonomic control and biochemical profile of animals exposed to a Western diet in the perinatal period.
Resumo: Introdução e objetivos: O consumo da dieta ocidental está associado ao surgimento de doenças cardiovasculares. O treinamento de resistência (TR) tem sido utilizado no tratamento destas ...doenças. O objetivo deste trabalho foi avaliar o efeito do TR sobre as alterações cardiometabólicas na prole de ratas expostas a dieta ocidental no período perinatal. Métodos: Ratas Wistar receberam dieta controle ou ocidental durante a gravidez e lactação. Os filhotes foram divididos em três grupos: Controle (C), dieta ocidental sedentário (OCS) e dieta ocidental + TR (OCTR). Aos 60 dias de vida, os animais iniciaram o protocolo de TR realizado cinco vezes por semana durante quatro semanas. Ao fim, foi registrada pressão arterial para análise da variabilidade da frequência cardíaca e sensibilidade do barorreflexo (SBR). Amostras de sangue foram coletadas para análise bioquímica. Resultados: O TR foi capaz de reduzir a pressão arterial, a modulação simpática vascular e aumentar a SBR. Houve melhoria no perfil bioquímico, com redução na glicemia de jejum, colesterol total e lipoproteínas de baixa densidade, além de aumento das lipoproteínas de alta densidade. Conclusão: O TR promoveu adaptações benéficas ao sistema cardiovascular, mediadas por ajustes nos mecanismos de controle autonômico e perfil bioquímico dos animais expostos à dieta ocidental no período perinatal. Abstract: Introduction and objectives: Consumption of a Western diet during the perinatal period is associated with development of cardiovascular disease. Resistance training (RT) has been used to treat cardiovascular disorders. The aim of this work was to assess the effect of RT on cardiometabolic disorders in rats exposed to a Western diet in the perinatal period. Methods: Female Wistar rats were fed with control or Western diet during pregnancy and lactation. The pups were divided into three groups: Control (C), Western Diet Sedentary (WDS) and Western Diet + RT (WDRT). At 60 days of age, all animals started the RT protocol (five times a week for four weeks). At the end, blood pressure was recorded for analysis of heart rate variability and baroreflex sensitivity (BRS). Blood samples were collected for biochemical analysis. Results: RT reduced blood pressure and vascular sympathetic modulation and increased BRS. There were improvements in biochemical profile, with reductions in fasting blood glucose, total cholesterol and low‐density lipoprotein, and an increase in high‐density lipoprotein. Conclusion: RT led to beneficial adaptations in the cardiovascular system, mediated by changes in the mechanisms of autonomic control and biochemical profile of animals exposed to a Western diet in the perinatal period. Palavras‐chave: Treinamento de resistência, Sistema nervoso autônomo, Dieta ocidental, Keywords: Resistance training, Autonomic nervous system, Western diet
AbstractIntroduction and ObjectivesConsumption of a Western diet during the perinatal period is associated with development of cardiovascular disease. Resistance training (RT) has been used to treat ...cardiovascular disorders. The aim of this work was to assess the effect of RT on cardiometabolic disorders in rats exposed to a Western diet in the perinatal period.MethodsFemale Wistar rats were fed with control or Western diet during pregnancy and lactation. The pups were divided into three groups: Control (C), Western Diet Sedentary (WDS) and Western Diet+RT (WDRT). At 60 days of age, all animals started the RT protocol (five times a week for four weeks). At the end, blood pressure was recorded for analysis of heart rate variability and baroreflex sensitivity (BRS). Blood samples were collected for biochemical analysis.ResultsRT reduced blood pressure and vascular sympathetic modulation and increased BRS. There were improvements in biochemical profile, with reductions in fasting blood glucose, total cholesterol and low-density lipoprotein, and an increase in high-density lipoprotein.ConclusionRT led to beneficial adaptations in the cardiovascular system, mediated by changes in the mechanisms of autonomic control and biochemical profile of animals exposed to a Western diet in the perinatal period.
The aim of this study was to investigate the wound healing activity of atranorin (AT) cream (Patented ‐ Brazil) on excision wounds. Excision wound: Seventy‐two male wistar rats were anesthetized and ...an excisional wound was performed. Then the rats were randomly assigned into 3 groups: CTR (untreated control group); AT1 (group treated with 1% AT ointment); and AT5 (group treated with 5% AT ointment). Six animals of each group were euthanized 3, 7, 14 or 21 days after the surgical procedures and the wounded areas were analyzed and removed. Serial histological sections were obtained and stained in histochemical techniques (Hematoxilin‐Eosin – HE – and Sirius red) and immunohistochemical techniques. Topical application of AT reduced wound areas, induced earlier granulation tissue formation, increased cell proliferation, improved collagenization and modulated the myofibroblasts differentiation when compared to control animals. It is suggested that atranorin modulates the wound healing process. This product may be quite useful in improving wound healing and a new biotechnological product with healing clinical application. Financial Support: FAPITEC/SE/Brazil; CNPQ/Brazil.
The aim of this study was to evaluate the effects of resistance training on blood pressure, ventricular repolarization, baroreflex sensitivity and cardiac autonomic balance in diabetic rats. The ...resistance training consisted of 3 sets of 10 repetitions performed at 40% of one repetition maximum test, 3 days/wk over 8 wks in squat‐training apparatus. Baroreflex sensitivity was analyzed by sequence method and cardiac autonomic balance by heart rate variability in the frequency domain. Diabetes increased glycemia (from 83 ± 8 to 381 ± 41 mg/dl, p<0.05), mean blood pressure (from 104.7 ± 5.4 to 125.1 ± 5.4 mmHg, p<0.05), QTc interval (from 4.4 ± 0.1 to 5.1 ± 0.1 ms, p<0.05), reduced baroreflex sensitivity (from 2.01 ± 0.3 to 0.38 ± 0.1 ms/mmHg, p<0.05) and impaired the cardiac autonomic balance. Resistance training was able to produce reduction on the glycemia (270 ± 17 mg/dl, p<0.05), prevented the increase of mean blood pressure (108 ± 3 mmHg, p<0.001) and QTc interval (4.6 ± 0.1 ms, p<0.01), the reduction of baroreflex sensitivity (2.63 ± 0.5 ms/mmHg, p<0.01) and disturbance on the cardiac autonomic balance. These results suggest that resistance training promotes a better glycemic control, prevents hypertension and improves baroreflex sensitivity and cardiac autonomic balance in diabetic rats.
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Insulin resistance (IR) precedes clinical manifestation of diseases such as diabetes mellitus and hypertension and is associated with increased sympathetic modulation. However, in the ...dexamethasone‐induced IR no changes have been noted in heart rate (HR). Therefore, we evaluated the heart chronotropic competence through beta‐adrenergic agonist stimulation in dexamethasone‐induced IR. Young male rats were divided into two groups: Control (CT)(n=6) and IR (n=6). CT and IR groups received vehicle or dexamethasone (DEX) (2mg/kg/day) i.p during seven days, respectively. To confirm Insulin resistance, insulin tolerance test (ITT) was performed after 6h fasted. Left femoral artery and vein were cannulated to measure blood pressure (2 kHz sampling rate) and isoproterenol (4μg/kg) administration respectively, 24h before evaluation. IR group presented attenuated decrease in glycaemia during ITT (CT: 42.5 ± 5 vs IR: 110.7 ± 14), hypertension (CT: 100 ± 2 vs IR: 135 ± 9 mmHg) without heart rate changes. In addition, increased cardiac sympathetic modulation by spectral analysis (LF/HF ratio CT: 0.52 ± 0.03 vs IR: 0.69 ± 0.04 un). After beta‐adrenergic stimulation IR group showed lower variation from basal level (ΔHR CT: 179 ± 4 vs IR: 78 ± 5 bpm). The reduction in heart chronotropic competence observed in this IR model could be a defense mechanism due cardiac sympathetic over activity.
Support or Funding Information
Fapitec‐SE; CAPES; CNPq.
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The aim of this study was to investigate the effect of moderate intensity strength exercise (SE) on autonomic balance and spontaneous barorreflex sensitivity (BRS) in rats. We used male ...Wistar rats (280–320g) that were divided into 2 groups (n=5 for each group): Sham‐(SG) and Trained‐Group (TG). TG rats were subjected to SE (1 set of 10 series with 10 repetitions) in a squat‐training apparatus. SG rats were sham trained. Before SE test, we determined the one‐repetition maximum (1RM), which means the maximum weight lifted by each animal. For this experiment we used sets of 60% of 1RM. The rats were anesthetized (sodium thiopental, 50 mg/kg, i.p.) and the right carotid was instrumented with a catheter for blood pressure (BP) and pulse interval recording. The variability of pulse interval, BP and BRS were analyzed. The SE was able to decrease the mean arterial pressure (MAP) 60 to 90 min (TG = 132 ± 2.7mmHg to 117 ± 1.6mmHg vs SG = 131 ± 2.6mmHg to 129 ± 1.7mmHg) after SE test. SE also reduced LFsys (25% lower) when compared to SG and increased BRS 45 to 90 min after exercise (TG=1.6±0.4mmHg/s to 2.4±0.6 mmHg/s vs SG= 1.9±0.1 mmHg/s to 1±0.07 mmHg/s). SE was able to increase LF/HF ratio (82%) and heart rate (7%). In summary moderate SE was able to reduce MAP despite higher cardiac sympathetic activity observed during all experimental period. Research support: Fapitec‐SE, CNPq, CAPES.
The aim of this study was to evaluate the effects of RT on glucose, lipids, urea, creatinine, PCRhs, fructosamine and blood TBARS, and mass indexes heart, liver and muscle in diabetic rats induced by ...alloxan (40 mg/kg, iv). For all experiments we used male Wistar rats weighing 200–300g. The animals were divided into 3 groups (n=8 for each group), sedentary control (SC), sedentary diabetic (SD) and trained diabetic (TD) rats. The RT was performed in a squat‐training apparatus using 3x10 repetitions, 3 times a week for 8 weeks After measurement of the maximum weight lifted (1RM) the training load was set at 40% of 1RM and adjusted each 15 days. At the end of the RT protocol or time control, the animals were anesthetized with sodium thiopental (45 mg/kg i.p.) and sample blood and tissues were obtained. The RT was able to reduce blood glucose (from 381 ± 42 to 270 ± 17 mg/dL), plasma TBARS (1.4 ± 0.02 to 0.2 ± 0.05 nmol/ml) and liver mass index (0.0467 ± 0.0036 to 0.0391 ± 0.0034), and to increase HDL (from 16.25 ± 2.25 to 36.6 ± 6.7) and plantar muscle mass index (from 0.00295 ± 0.00026 to 0.00377 ± 0.00005) in relation to the SD. These results demonstrate that the low intensity RT is a useful non‐pharmacological intervention to treatment of metabolic complications from diabetes. Research Support: CAPES, Fapitec‐SE.
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