The RHIC Spin Program Overview Bazilevsky, A.
Journal of physics. Conference series,
02/2016, Letnik:
678, Številka:
1
Journal Article
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After more than a decade of RHIC running as a polarized proton collider, we summarize recent achievements of the RHIC spin program and their impact on our understanding of the nucleon's spin ...structure, i.e. the individual parton (quarks and gluons) contributions to the helicity structure of the nucleon, and to understand the origin of the transverse spin phenomena. Open questions are identified and a suite of future measurements with polarized beams at RHIC to address them is laid out.
Over the last decade, numerous histone acyl post-translational modifications (acyl-PTMs) have been discovered, of which the functional significance is still under intense study. Here, we use ...high-resolution mass spectrometry to accurately quantify eight acyl-PTMs in vivo and after in vitro enzymatic assays. We assess the ability of seven histone acetyltransferases (HATs) to catalyze acylations on histones in vitro using short-chain acyl-CoA donors, proving that they are less efficient towards larger acyl-CoAs. We also observe that acyl-CoAs can acylate histones through non-enzymatic mechanisms. Using integrated metabolomic and proteomic approaches, we achieve high correlation (R
> 0.99) between the abundance of acyl-CoAs and their corresponding acyl-PTMs. Moreover, we observe a dose-dependent increase in histone acyl-PTM abundances in response to acyl-CoA supplementation in in nucleo reactions. This study represents a comprehensive profiling of scarcely investigated low-abundance histone marks, revealing that concentrations of acyl-CoAs affect histone acyl-PTM abundances by both enzymatic and non-enzymatic mechanisms.
This work examines the release of a model water-soluble compound from electrospun polymer nanofiber assemblies. Such release attracts attention in relation to biomedical applications, such as ...controlled drug delivery. It is also important for stem cell attachment and differentiation on biocompatible electrospun nanofiber scaffolds containing growth factors, which have been encapsulated by means of electrospinning. Typically, the release mechanism has been attributed to solid-state diffusion of the encapsulated compound from the fibers into the surrounding aqueous bath. Under this assumption, a 100% release of the encapsulated compound is expected in a certain (long) time. The present work focuses on certain cases where complete release does not happen, which suggests that solid-state diffusion may not be the primary mechanism at play. We show that in such cases the release rate can be explained by desorption of the embedded compound from nanopores in the fibers or from the outer surface of the fibers in contact with the water bath. After release, the water-soluble compound rapidly diffuses in water, whereas the release rate is determined by the limiting desorption stage. A model system of Rhodamine 610 chloride fluorescent dye embedded in electrospun monolithic poly(methylmethacrylate) (PMMA) or poly(caprolactone) (PCL) nanofibers, in nanofibers electrospun from PMMA/PCL blends, or in core−shell PMMA/PCL nanofibers is studied. Both the experimental results and theory point at the abovementioned desorption-related mechanism, and the predicted characteristic time, release rate, and effective diffusion coefficient agree fairly well with the experimental data. A practically important outcome of this surface release mechanism is that only the compound on the fiber and pore surfaces can be released, whereas the material encapsulated in the bulk cannot be freed within the time scales characteristic of the present experiments (days to months). Consequently, in such cases, complete release is impossible. We also demonstrate how the release rate can be manipulated by the polymer content and molecular weight affecting nanoporosity and the desorption enthalpy, as well as by the nanofiber structure (monolithic fibers, fibers from polymer blends, and core−shell fibers). In particular, it is shown that, by manipulating the above parameters, release times from tens of hours to months can be attained.
The transverse impact of a pulsed water microjet on individual cylindrical fibers is studied. The stages of ejection, breakup, and collision of the microjet were recorded by high-speed photography. A ...significant deceleration of the microjet by the fiber and its splitting into two parts were revealed. The mechanisms of the observed phenomena and the influence of various factors are discussed.
Aims. We develop a physical model to explain the potent outbursts that occurred in the fractured terrain of comet 67P near perihelion, and predict its temporal characteristics. Methods. The ...feasibility of the proposed mechanism is studied using a numerical model accounting for the relevant microscopic/macroscopic processes. We rely on the thermophysical, compositional, and geo-morphological data from the published measurements of respective instruments on board Rosetta. Results. The key idea of this novel mechanism is built around observations of fractures/cracks in the region of interest. It is argued that as the stresses on the nucleus increased during the perihelion approach, a crack deepening event occurred reaching the deeper material containing super-volatile ices in equilibrium with the surrounding. This sudden opening lead to a violent sublimation of the super-volatile ices. The time scales and mass release of this process are modeled and reported. In our modeling we pay attention to the question of the existence of super-volatile ices in the deeper interior for a long time, and the thermal equilibrium in the interior. Conclusions. The deepening of pre-existing cracks (fracture) into the material containing highly volatile ices can explain the observed outburst features. The sudden disequilibration of the steady-state reservoir of highly volatile ices results in a violent release of gas and dust. The proposed mechanism also explains the rapid shut down of this activity in accordance with the observations. The proposed mechanism is independent of solar illumination history of a given region, or the pre-existance of large sealed nucleus cavities.
ATP-citrate lyase (ACLY) synthesizes cytosolic acetyl coenzyme A (acetyl-CoA), a fundamental cellular building block. Accordingly, aberrant ACLY activity is observed in many diseases. Here we report ...cryo-EM structures of human ACLY, alone or bound to substrates or products. ACLY forms a homotetramer with a rigid citrate synthase homology (CSH) module, flanked by four flexible acetyl-CoA synthetase homology (ASH) domains; CoA is bound at the CSH-ASH interface in mutually exclusive productive or unproductive conformations. The structure of a catalytic mutant of ACLY in the presence of ATP, citrate and CoA substrates reveals a phospho-citryl-CoA intermediate in the ASH domain. ACLY with acetyl-CoA and oxaloacetate products shows the products bound in the ASH domain, with an additional oxaloacetate in the CSH domain, which could function in ACLY autoinhibition. These structures, which are supported by biochemical and biophysical data, challenge previous proposals of the ACLY catalytic mechanism and suggest additional therapeutic possibilities for ACLY-associated metabolic disorders.
We derive the effect of 3-dimensional polarization profiles on the measured polarization in polarimeters, as well as the observed polarization and the polarization-weighted luminosity (figure of ...merit) in single and double spin measurements in colliding beam experiments.
ATP-citrate lyase (ACLY) is a major source of nucleocytosolic acetyl-CoA, a fundamental building block of carbon metabolism in eukaryotes. ACLY is aberrantly regulated in many cancers, cardiovascular ...disease, and metabolic disorders. However, the molecular mechanisms determining ACLY activity and function are unclear. To this end, we investigated the role of the uncharacterized ACLY C-terminal citrate synthase homology domain in the mechanism of acetyl-CoA formation. Using recombinant, purified ACLY and a suite of biochemical and biophysical approaches, including analytical ultracentrifugation, dynamic light scattering, and thermal stability assays, we demonstrated that the C terminus maintains ACLY tetramerization, a conserved and essential quaternary structure in vitro and likely also in vivo. Furthermore, we show that the C terminus, only in the context of the full-length enzyme, is necessary for full ACLY binding to CoA. Together, we demonstrate that ACLY forms a homotetramer through the C terminus to facilitate CoA binding and acetyl-CoA production. Our findings highlight a novel and unique role of the C-terminal citrate synthase homology domain in ACLY function and catalysis, adding to the understanding of the molecular basis for acetyl-CoA synthesis by ACLY. This newly discovered means of ACLY regulation has implications for the development of novel ACLY modulators to target acetyl-CoA–dependent cellular processes for potential therapeutic use.
A method for the on-line testing of small volumes of biological fluids is presented. The method is based on the ability of some liquids to form, when stretched, long thinning filaments, liquid ...bridges. Measuring the filament thinning rate makes it possible to determine the rheological parameters of the fluid. The method was tested on synthetic polymer solutions and biological fluids, such as saliva, bronchial sputum, follicular fluid, and chicken egg albumen and yolk. The results evidence that this technique can be used in various biomechanical applications, in particular, for disease diagnostics and treatment monitoring.
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