To better predict the ecological and evolutionary effects of the emerging biodiversity crisis in the modern oceans, we compared the association between extinction threat and ecological traits in ...modern marine animals to associations observed during past extinction events using a database of 2497 marine vertebrate and mollusc genera. We find that extinction threat in the modern oceans is strongly associated with large body size, whereas past extinction events were either nonselective or preferentially removed smaller-bodied taxa. Pelagic animals were victimized more than benthic animals during previous mass extinctions but are not preferentially threatened in the modern ocean. The differential importance of large-bodied animals to ecosystem function portends greater future ecological disruption than that caused by similar levels of taxonomic loss in past mass extinction events.
During the past 541 million years, marine animals underwent three intervals of diversification (early Cambrian, Ordovician, Cretaceous-Cenozoic) separated by nondirectional fluctuation, suggesting ...diversity-dependent dynamics with the equilibrium diversity shifting through time. Changes in factors such as shallow-marine habitat area and climate appear to have modulated the nondirectional fluctuations. Directional increases in diversity are best explained by evolutionary innovations in marine animals and primary producers coupled with stepwise increases in the availability of food and oxygen. Increasing intensity of biotic interactions such as predation and disturbance may have led to positive feedbacks on diversification as ecosystems became more complex. Important areas for further research include improving the geographic coverage and temporal resolution of paleontological data sets, as well as deepening our understanding of Earth system evolution and the physiological and ecological traits that modulated organismal responses to environmental change.
The aim of this updated statement is to provide comprehensive and timely evidence-based recommendations on the prevention of ischemic stroke among survivors of ischemic stroke or transient ischemic ...attack. Evidence-based recommendations are included for the control of risk factors, interventional approaches for atherosclerotic disease, antithrombotic treatments for cardioembolism, and the use of antiplatelet agents for noncardioembolic stroke. Further recommendations are provided for the prevention of recurrent stroke in a variety of other specific circumstances, including arterial dissections; patent foramen ovale; hyperhomocysteinemia; hypercoagulable states; sickle cell disease; cerebral venous sinus thrombosis; stroke among women, particularly with regard to pregnancy and the use of postmenopausal hormones; the use of anticoagulation after cerebral hemorrhage; and special approaches to the implementation of guidelines and their use in high-risk populations.
Pseudomonas aeruginosa strain PA14 is a multi-host pathogen that infects plants, nematodes, insects, and vertebrates. Many PA14 factors are required for virulence in more than one of these hosts. ...Noting that plants have a fundamentally different cellular architecture from animals, we sought to identify PA14 factors that are specifically required for plant pathogenesis. We show that synthesis by PA14 of the disaccharide trehalose is required for pathogenesis in Arabidopsis, but not in nematodes, insects, or mice. In-frame deletion of two closely-linked predicted trehalose biosynthetic operons, treYZ and treS, decreased growth in Arabidopsis leaves about 50 fold. Exogenously co-inoculated trehalose, ammonium, or nitrate, but not glucose, sulfate, or phosphate suppressed the phenotype of the double ΔtreYZΔtreS mutant. Exogenous trehalose or ammonium nitrate does not suppress the growth defect of the double ΔtreYZΔtreS mutant by suppressing the plant defense response. Trehalose also does not function intracellularly in P. aeruginosa to ameliorate a variety of stresses, but most likely functions extracellularly, because wild-type PA14 rescued the in vivo growth defect of the ΔtreYZΔtreS in trans. Surprisingly, the growth defect of the double ΔtreYZΔtreS double mutant was suppressed by various Arabidopsis cell wall mutants that affect xyloglucan synthesis, including an xxt1xxt2 double mutant that completely lacks xyloglucan, even though xyloglucan mutants are not more susceptible to pathogens and respond like wild-type plants to immune elicitors. An explanation of our data is that trehalose functions to promote the acquisition of nitrogen-containing nutrients in a process that involves the xyloglucan component of the plant cell wall, thereby allowing P. aeruginosa to replicate in the intercellular spaces in a leaf. This work shows how P. aeruginosa, a multi-host opportunistic pathogen, has repurposed a highly conserved "house-keeping" anabolic pathway (trehalose biosynthesis) as a potent virulence factor that allows it to replicate in the intercellular environment of a leaf.
Abstract
Young planets provide a window into the early stages and evolution of planetary systems. Ideal planets for such research are in coeval associations, where the parent population can precisely ...determine their ages. We describe a young association (MELANGE-3) in the Kepler field, which harbors two transiting planetary systems (KOI-3876 and Kepler-970). We identify MELANGE-3 by searching for kinematic and spatial overdensities around Kepler planet hosts with high levels of lithium. To determine the age and membership of MELANGE-3, we combine new high-resolution spectra with archival light curves, velocities, and astrometry of stars near KOI-3876 spatially and kinematically. We use the resulting rotation sequence, lithium levels, and color–magnitude diagram of candidate members to confirm the presence of a coeval 105 ± 10 Myr population. MELANGE-3 may be part of the recently identified Theia 316 stream. For the two exoplanet systems, we revise the stellar and planetary parameters, taking into account the newly determined age. Fitting the 4.5 yr Kepler light curves, we find that KOI-3876b is a 2.0 ± 0.1
R
⊕
planet on a 19.58 day orbit, while Kepler-970 b is a 2.8 ± 0.2
R
⊕
planet on a 16.73 day orbit. KOI-3876 was previously flagged as an eclipsing binary, which we rule out using radial velocities from APOGEE and statistically validate the signal as planetary in origin. Given its overlap with the Kepler field, MELANGE-3 is valuable for studies of spot evolution on year timescales, and both planets contribute to the growing work on transiting planets in young stellar associations.
Ecological differentiation is correlated with taxonomic diversity in many clades, and ecological divergence is often assumed to be a cause and/or consequence of high speciation rate. However, an ...analysis of 30,074 genera of living marine animals and 19,992 genera of fossil marine animals indicates that greater ecological differentiation in the modern oceans is actually associated with lower rates of origination over evolutionary time. Ecologically differentiated clades became taxonomically diverse over time because they were better buffered against extinction, particularly during mass extinctions, which primarily affected genus-rich, ecologically homogeneous clades. The relationship between ecological differentiation and taxonomic richness was weak early in the evolution of animals but has strengthened over geological time as successive extinction events reshaped the marine fauna.
Abstract
The dispersed remnants of stellar nurseries, stellar associations, provide unparalleled samples of coeval stars critical for studies of stellar and planetary formation and evolution. The ...Carina Stellar Association is one of the closest stellar associations to Earth, and yet measurements of its age have varied from 13 to 45 Myr. We aim to update the age of Carina using the lithium depletion boundary (LDB) method. We obtain new measurements of the Li 6708 Å absorption feature in likely members using optical spectra from the Goodman High Throughput Spectrograph on SOAR and NRES on LCO. We detect the depletion boundary at
M
K
≃ 6.8 (M5). This age is consistent within uncertainties across six different models, including those that account for magnetic fields and spots. We also estimate the age through analysis of the group’s overall variability, and by comparing the association members’ color–magnitude diagram to stellar evolutionary models using a Gaussian Mixture Model, recovering ages consistent with the LDB. Combining these age measures we obtain an age for the Carina association of
41
−
5
+
3
Myr. The resulting age agrees with the older end of previous age measurements and is consistent with the lithium depletion age for the neighboring Tucana-Horologium moving group.
Abstract We report the discovery and characterization of a nearby (∼85 pc), older (27 ± 3 Myr), distributed stellar population near Lower Centaurus Crux (LCC), initially identified by searching for ...stars comoving with a candidate transiting planet from TESS (HD 109833; TOI 1097). We determine the association membership using Gaia kinematics, color–magnitude information, and rotation periods of candidate members. We measure its age using isochrones, gyrochronology, and Li depletion. While the association is near known populations of LCC, we find that it is older than any previously found LCC subgroup (10–16 Myr), and distinct in both position and velocity. In addition to the candidate planets around HD 109833, the association contains four directly imaged planetary-mass companions around three stars, YSES-1, YSES-2, and HD 95086, all of which were previously assigned membership in the younger LCC. Using the Notch pipeline, we identify a second candidate transiting planet around HD 109833. We use a suite of ground-based follow-up observations to validate the two transit signals as planetary in nature. HD 109833 b and c join the small but growing population of <100 Myr transiting planets from TESS. HD 109833 has a rotation period and Li abundance indicative of a young age (≲100 Myr), but a position and velocity on the outskirts of the new population, lower Li levels than similar members, and a color–magnitude diagram position below model predictions for 27 Myr. So, we cannot reject the possibility that HD 109833 is a young field star coincidentally nearby the population.
The most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD) is an expanded G
C
RNA repeat r(G
C
)
in chromosome 9 open reading frame 72 (
), which elicits ...pathology through several mechanisms. Here, we developed and characterized a small molecule for targeted degradation of r(G
C
)
. The compound was able to selectively bind r(G
C
)
’s structure and to assemble an endogenous nuclease onto the target, provoking removal of the transcript by native RNA quality control mechanisms. In c9ALS patient–derived spinal neurons, the compound selectively degraded the mutant
allele with limited off-targets and reduced quantities of toxic dipeptide repeat proteins (DPRs) translated from r(G
C
)
. In vivo work in a rodent model showed that abundance of both the mutant allele harboring the repeat expansion and DPRs were selectively reduced by this compound. These results demonstrate that targeted small-molecule degradation of r(G
C
)
is a strategy for mitigating c9ALS/FTD-associated pathologies and studying disease-associated pathways in preclinical models.
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