The cell wall is a stress-bearing structure and a unifying trait in bacteria. Without exception, synthesis of the cell wall involves formation of the precursor molecule lipid II by the activity of ...the essential biosynthetic enzyme MurG, which is encoded in the division and cell wall synthesis (
) gene cluster. Here, we present the discovery of a cell wall enzyme that can substitute for MurG. A mutant of
lacking a significant part of the
cluster, including
, surprisingly produced lipid II and wild-type peptidoglycan. Genomic analysis identified a distant
homologue, which encodes a putative enzyme that shares only around 31% amino acid sequence identity with MurG. We show that this enzyme can replace the canonical MurG, and we therefore designated it MglA. Orthologues of
are present in 38% of all genomes of
and members of the sister genus
CRISPR interference experiments showed that
can also functionally replace
in
, thus validating its bioactivity and demonstrating that it is active in multiple genera. All together, these results identify MglA as a bona fide lipid II synthase, thus demonstrating plasticity in cell wall synthesis.
Almost all bacteria are surrounded by a cell wall, which protects cells from environmental harm. Formation of the cell wall requires the precursor molecule lipid II, which in bacteria is universally synthesized by the conserved and essential lipid II synthase MurG. We here exploit the unique ability of an actinobacterial strain capable of growing with or without its cell wall to discover an alternative lipid II synthase, MglA. Although this enzyme bears only weak sequence similarity to MurG, it can functionally replace MurG and can even do so in organisms that naturally have only a canonical MurG. The observation that MglA proteins are found in many actinobacteria highlights the plasticity in cell wall synthesis in these bacteria and demonstrates that important new cell wall biosynthetic enzymes remain to be discovered.
A new method to tag the barium daughter in the double-beta decay of ^{136}Xe is reported. Using the technique of single molecule fluorescent imaging (SMFI), individual barium dication (Ba^{++}) ...resolution at a transparent scanning surface is demonstrated. A single-step photobleach confirms the single ion interpretation. Individual ions are localized with superresolution (∼2 nm), and detected with a statistical significance of 12.9σ over backgrounds. This lays the foundation for a new and potentially background-free neutrinoless double-beta decay technology, based on SMFI coupled to high pressure xenon gas time projection chambers.
A
bstract
NEXT-100 is an electroluminescent high-pressure xenon gas time projection chamber that will search for the neutrinoless double beta (0
νββ
) decay of
136
Xe. The detector possesses two ...features of great value for 0
νββ
searches: energy resolution better than 1% FWHM at the
Q
value of
136
Xe and track reconstruction for the discrimination of signal and background events. This combination results in excellent sensitivity, as discussed in this paper. Material-screening measurements and a detailed Monte Carlo detector simulation predict a background rate for NEXT-100 of at most 4 × 10
−4
counts keV
−1
kg
−1
yr
−1
. Accordingly, the detector will reach a sensitivity to the 0
νββ
-decay half-life of 2.8 × 10
25
years (90% CL) for an exposure of 100 kg·year, or 6.0 × 10
25
years after a run of 3 effective years.
We introduce a simulation framework for the transport of high and low energy electrons in xenon-based optical time projection chambers (OTPCs). The simulation relies on elementary cross sections ...(electron–atom and electron–molecule) and incorporates, in order to compute the gas scintillation, the reaction/quenching rates (atom–atom and atom–molecule) of the first 41 excited states of xenon and the relevant associated excimers, together with their radiative cascade. The results compare positively with observations made in pure xenon and its mixtures with CO2 and CF4 in a range of pressures from 0.1 to 10 bar. This work sheds some light on the elementary processes responsible for the primary and secondary xenon-scintillation mechanisms in the presence of additives, that are of interest to the OTPC technology.
Xe–CO2 mixtures are important alternatives to pure xenon in Time Projection Chambers (TPC) based on secondary scintillation (electroluminescence) signal amplification with applications in the ...important field of rare event detection such as directional dark matter, double electron capture and double beta decay detection. The addition of CO2 to pure xenon at the level of 0.05–0.1% can reduce significantly the scale of electron diffusion from 10 mm/m to 2.5 mm/m, with high impact on the discrimination efficiency of the events through pattern recognition of the topology of primary ionization trails. We have measured the electroluminescence (EL) yield of Xe–CO2 mixtures, with sub-percent CO2 concentrations. We demonstrate that the EL production is still high in these mixtures, 70% and 35% relative to that produced in pure xenon, for CO2 concentrations around 0.05% and 0.1%, respectively. The contribution of the statistical fluctuations in EL production to the energy resolution increases with increasing CO2 concentration, being smaller than the contribution of the Fano factor for concentrations below 0.1% CO2.
A
bstract
Double electron capture by proton-rich nuclei is a second-order nuclear process analogous to double beta decay. Despite their similarities, the decay signature is quite different, ...potentially providing a new channel to measure the hypothesized neutrinoless mode of these decays. The Standard-Model-allowed two-neutrino double electron capture (2
νEC EC
) has been predicted for a number of isotopes, but only observed in
78
Kr,
130
Ba and, recently,
124
Xe. The sensitivity to this decay establishes a benchmark for the ultimate experimental goal, namely the potential to discover also the lepton-number-violating neutrinoless version of this process, 0
νEC EC
. Here we report on the current sensitivity of the NEXT-White detector to
124
Xe 2
νEC EC
and on the extrapolation to NEXT-100. Using simulated data for the 2
νEC EC
signal and real data from NEXT-White operated with
124
Xe-depleted gas as background, we define an optimal event selection that maximizes the NEXT-White sensitivity. We estimate that, for NEXT-100 operated with xenon gas isotopically enriched with 1 kg of
124
Xe and for a 5-year run, a sensitivity to the 2
νEC EC
half-life of 6
×
10
22
y (at 90% confidence level) or better can be reached.
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