Phys. Lett. B 820 (2021) 136557 Using $e^+e^-$ collision data at ten center-of-mass energies between 2.644
and 3.080 GeV collected with the BESIII detector at BEPCII and corresponding to
an ...integrated luminosity of about 500 pb$^{-1}$, we measure the cross sections
and effective form factors for the process
$e^{+}e^{-}\rightarrow\Xi^{0}\bar{\Xi}^{0}$ utilizing a single-tag method. A
fit to the cross section of $e^{+}e^{-}\rightarrow\Xi^{0}\bar{\Xi}^{0}$ with a
pQCD-driven power function is performed, from which no significant resonance or
threshold enhancement is observed. In addition, the ratio of cross sections for
the processes $e^{+}e^{-}\rightarrow\Xi^{-}\bar{\Xi}^{+}$ and
$\Xi^{0}\bar{\Xi}^{0}$ is calculated using recent BESIII measurement and is
found to be compatible with expectation from isospin symmetry.
Our previous study indicated that CD177+ neutrophil activation has a vital role in the pathogenesis of biliary atresia (BA), which is partially ameliorated by N-acetylcysteine (NAC) treatment. Here, ...we evaluated the clinical efficacy of NAC treatment and profiled liver-resident immune cells via single cell RNA-sequencing (scRNA-seq) analysis to provide a comprehensive immune landscape of NAC-derived immune regulation.
A pilot clinical study was conducted to evaluate the potential effects of intravenous NAC treatment on infants with BA, and a 3-month follow-up was carried out to assess treatment efficacy. scRNA-seq analysis of liver CD45+ immune cells in the control (n = 4), BA (n = 6), and BA + NAC (n = 6) groups was performed and the effects on innate cells, including neutrophil and monocyte–macrophage subsets, and lymphoid cells were evaluated.
Intravenous NAC treatment demonstrated beneficial efficacy for infants with BA by improving bilirubin metabolism and bile acid flow. Two hepatic neutrophil subsets of innate cells were identified by scRNA-seq analysis. NAC treatment suppressed oxidative phosphorylation and reactive oxygen species production in immature neutrophils, which were transcriptionally and functionally similar to CD177+ neutrophils. We also observed the suppression of hepatic monocyte-mediated inflammation, decreased levels of oxidative phosphorylation, and M1 polarisation in Kupffer-like macrophages by NAC. In lymphoid cells, enhancement of humoral immune responses and attenuation of cellular immune responses were observed after NAC treatment. Moreover, cell–cell interaction analysis showed that innate/adaptive proinflammatory responses were downregulated by NAC.
Our clinical and scRNA-seq data demonstrated that intravenous NAC treatment partially reversed liver immune dysfunction, alleviated the proinflammatory responses in BA by targeting innate cells, and exhibited beneficial clinical efficacy.
BA is a serious liver disease that affects newborns and has no effective drug treatment. In this study, scRNA-seq showed that NAC treatment can partially reverse the immune dysfunction of neutrophil extracellular trap-releasing CD177+ neutrophils and Kupffer cells, and lower the inflammatory responses of other innate immune cells in BA. In consequence, intravenous NAC treatment improved the clinical outcomes of patients with BA in term of bilirubin metabolism.
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•Intravenous NAC treatment improves bilirubin metabolism and bile acid flow in patients with BA.•NAC suppresses CD177+ hepatic immature neutrophil oxidative phosphorylation and reactive oxygen species production in BA.•NAC alleviates monocyte-mediated inflammation and reverses hepatic macrophage dysfunction in BA.•NAC downregulates innate/adaptive proinflammatory responses via cell–cell interactions in BA.
