Administration of drugs via the buccal route has attracted much attention in recent years. However, developing systems with satisfactory adhesion under wet conditions and adequate drug ...bioavailability still remains a challenge. Here, we propose a mussel-inspired mucoadhesive film. Ex vivo models show that this film can achieve strong adhesion to wet buccal tissues (up to 38.72 ± 10.94 kPa). We also demonstrate that the adhesion mechanism of this film relies on both physical association and covalent bonding between the film and mucus. Additionally, the film with incorporated polydopamine nanoparticles shows superior advantages for transport across the mucosal barrier, with improved drug bioavailability (~3.5-fold greater than observed with oral delivery) and therapeutic efficacy in oral mucositis models (~6.0-fold improvement in wound closure at day 5 compared with that observed with no treatment). We anticipate that this platform might aid the development of tissue adhesives and inspire the design of nanoparticle-based buccal delivery systems.
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•A framework for assessing coordinative relationship was established.•Coordinative relationship was quantified by using coupling coordinating degree model.•The CES of each city in ...ARDUA had deteriorated during the study period.•The coordinative relationship in ARDUA was always in a state of mild incoordination.•The coordinative relationship showed an obvious regional difference.
Understanding the coordinative relationship between urbanization and ecosystem services (ESs) has a great significance in sustainable development in urban agglomeration. In this paper, InVEST model was firstly used to assess the spatio-temporal evaluation of four types of ESs (water yield, carbon storage, soil retention and nitrogen export) in the Yangtze River Delta Urban Agglomerations (YRDUA) of China from 2000 to 2020. Then comprehensive evaluation index system of urbanization was established from the multi-dimensions of economy, population and land to analyze the urbanization level during the study period. Finally, a comprehensive ecosystem service (CES) index was developed to explore the coordinative relationship between ESs and urbanization by using coupling coordinating degree model (CCDM). The results revealed that: 1) Urbanization has led to greatly land use/ land cover change, which caused the degradation of carbon storage and water purification, and the improvement of water yield and soil retention. The higher water yield and nitrogen export mainly clustered around urban areas, while higher carbon storage and soil retention were distributed in forest-rich cities in the Zhejiang and the southern of Anhui. There were positive correlations between different ESs. And the CES of cities in the YRDUA had deteriorated during the study period. 2) The urbanization levels of almost all cities showed an overall upward trend, with Shanghai the highest urbanization, followed by Nanjing and Hangzhou, and rapid urban expansion mainly occurred in developed urban areas. 3) The coordinative relationship between CES and urbanization in the YRDUA was always in mild incoordination, showing an obvious spatial distribution pattern of “higher in the south, but lower in the north”. The coordinative relationship between CES and urbanization in most cities in the north of YRDUA has not achieved coordination development, with the state of moderate or mild incoordination, while the cities in the south has attained coordination development, with the state of primary or even superior coordination. Therefore, our study suggested that differentiated urban space management and control policies should be made for the YRDUA to improve the overall coordinative relationship between CES and urbanization.
Due to their high specific surface area, graphene oxide and graphene oxide-base nanoparticles have great potential both in dual-drug delivery and combination chemotherapy. Herein, we developed ...cisplatin (Pt) and doxorubicin (DOX) dual-drug-loaded PEGylated nano-graphene oxide (pGO) to facilitate combined chemotherapy in one system. In this study, nano-sized pGO-Pt/DOX ranged around 161.50 nm was fabricated and characterized using zeta-potential, AFM, TEM, Raman, UV-Vis, and FTIR analyses. The drug delivery efficacy of Pt was enhanced through the introduction of pGO, and the final weight ratio of DOX: Pt: pGO was optimized to 0.376: 0.376: 1. In vitro studies revealed that pGO-Pt/DOX nanoparticles could be effectively delivered into tumor cells, in which they induced prominent cell apoptosis and necrosis and exhibited higher growth inhibition than the single drug delivery system or free drugs. The pGO-Pt/DOX induced the most prominent cancer cell apoptosis and necrosis rate with 18.6%, which was observed almost 2 times higher than that of pGO-Pt or pGO-DOX groups. in the apoptosis and necrotic quadrants In vivo data confirmed that the pGO-Pt/DOX dual-drug delivery system attenuated the toxicity of Pt and DOX to normal organs compared to free drugs. The tumor inhibition data, histopathology observations, and immunohistochemical staining confirmed that the dual-drug delivery system presented a better anticancer effect than free drugs. These results clearly indicated that the pGO-Pt/DOX dual-drug delivery system provided the means for combination drug delivery in cancer treatment.
High-density human activities have rapidly changed the pattern of urban land use/land cover (LULC), affecting the supply of urban ecosystem services (ESs). This poses a challenge to the balance of ...urban development and ecological protection, which may be more serious in ecologically fragile regions. This study identified key LULC change (LULCC) impacts on ESs in Ordos, an ecologically fragile region in Northwest China. The urbanization process of Ordos will continue in 2030 under the business-as-usual scenario due to its strategic positioning in the National Energy Base of China. The future LULC simulation results showed that developed land in eastern Ordos will continue to expand in 2030, and more forest and grassland will appear in western Ordos as a result of ecological restoration. The results of the InVEST model in calculating four important ESs showed that the ES supply in the densely populated areas (eastern Ordos) has declined, and more attention must be given to natural vegetation protection in the urbanization process. Although carbon storage in the western region has increased due to afforestation, this also reduces the water yield supply, which may exacerbate the water shortage in Ordos. Supported by this framework, more sustainable urban land use management can be undertaken to balance the conflict between ecological protection and urbanization. This will contribute to regional ecological health and sustainable urban development.
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•Ecosystem services (ESs) need specific attention in ecologically fragile regions.•Urbanization will continue in 2030 under the business-as-usual scenario in Ordos.•Beneficial ESs declined due to transfer from natural vegetation to developed land.•Ecological protection is particularly important in densely populated eastern counties.•Afforestation may reduce water yield which is challenging for water-scarce cities.
Bone tissue engineering commonly encompasses the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the propagation of cells to regenerate damaged tissues or organs. ...3D printing technology has been extensively applied to allow direct 3D scaffolds manufacturing. Polycaprolactone (PCL) has been widely used in the fabrication of 3D scaffolds in the field of bone tissue engineering due to its advantages such as good biocompatibility, slow degradation rate, the less acidic breakdown products in comparison to other polyesters, and the potential for loadbearing applications. PCL can be blended with a variety of polymers and hydrogels to improve its properties or to introduce new PCL-based composites. This paper describes the PCL used in developing state of the art of scaffolds for bone tissue engineering. In this review, we provide an overview of the 3D printing techniques for the fabrication of PCL-based composite scaffolds and recent studies on applications in different clinical situations. For instance, PCL-based composite scaffolds were used as an implant surgical guide in dental treatment. Furthermore, future trend and potential clinical translations will be discussed.
In recent years, the enormous losses caused by urban surface deformation have received more and more attention. Traditional geodetic techniques are point-based measurements, which have limitations in ...using traditional geodetic techniques to detect and monitor in areas where geological disasters occur. Therefore, we chose Interferometric Synthetic Aperture Radar (InSAR) technology to study the surface deformation in urban areas. In this research, we discovered the land subsidence phenomenon using InSAR and Global Navigation Satellite System (GNSS) technology. Two different kinds of time-series InSAR (TS-InSAR) methods: Small BAseline Subset (SBAS) and the Permanent Scatterer InSAR (PSI) process were executed on a dataset with 31 Sentinel-1A Synthetic Aperture Radar (SAR) images. We generated the surface deformation field of Shenzhen, China and Hong Kong Special Administrative Region (HKSAR). The time series of the 3d variation of the reference station network located in the HKSAR was generated at the same time. We compare the characteristics and advantages of PSI, SBAS, and GNSS in the study area. We mainly focus on the variety along the coastline area. From the results generated by SBAS and PSI techniques, we discovered the occurrence of significant subsidence phenomenon in the land reclamation area, especially in the metro construction area and the buildings with a shallow foundation located in the land reclamation area.
The sustainability of rural development, both economic and environmental, has been increasingly linking to local food, which plays an indispensable role by preserving traditional culture, attracting ...tourists, and supporting the regional economy. However, the authenticity and quality of local food have not been fully convinced as competitive advantages by most practitioners. Little is known about how authenticity affects quality attributes, tourist satisfaction, and tourist loyalty. Thus, this study examines the role of authenticity in the quality–satisfaction–loyalty framework. The field research was performed in Shunde County, Guangdong Province, China. The results challenge the traditional view of quality attributes by highlighting that authenticity is a key antecedent to the quality–satisfaction–loyalty framework of food tourism. In contrast, the relationships among quality attributes, tourist satisfaction, and tourist loyalty are contingent on the extent to which food tourists perceive the authenticity of rural local food.
•X-ray diffraction (XRD) was used to study the composition of corrosion products.•Smart aggregate (SA) technique was applied to monitor inner surface cracking.•Digital image correlation (DIC) method ...captured outer surface cracking.•Critical corrosion degree under different current density levels were predicted.•Two-stage model outperformed three-stage model to describe corrosion process.
Accelerated corrosion by means of impressed current technique is widely adopted to investigate durability problems of reinforced concrete structures caused by reinforcement corrosion. In this paper, the influence of the impressed current density level between 50 and 300 μA/cm2 on the corrosion induced cracking of concrete cover was experimentally studied. X-ray diffraction (XRD) method was used to determine the compositions and expansion coefficient of corrosion products under different current density levels. Smart aggregate (SA) and digital image correlation (DIC) techniques were applied to monitor the inner and outer surface cracking of reinforced concrete specimens, respectively, which will further determine the corresponding critical corrosion degrees. The experimental results indicate that the expansion coefficient of steel corrosion products decreased as the impressed current density increased, resulting in the increase of corrosion degree at inner and outer surface cracking of concrete cover. In addition, the existing numerical approaches based on three-stage model or two-stage model were conducted to predict the critical corrosion degree at different current density level. The comparison of simulation results with test results shows that the corrosion products filling into the pores happened together with the corrosion layer accumulating, which means the two-stage model outperformed three-stage model to describe the cracking process of concrete cover in the accelerated corrosion test.
Objective This study aimed to evaluate the oncological and reproductive outcomes of fertility-preserving re-treatment in progestin-resistant endometrial carcinoma (EC) and atypical endometrial ...hyperplasia (AEH) women who desire to maintain their fertility. Methods Our study included 61 progestin-resistant EC/AEH patients. These patients underwent treatment with gonadotropin-releasing hormone agonist (GnRHa) solely or a combination of GnRHa with levonorgestrel-releasing intrauterine system (LNG-IUD) or aromatase inhibitor (AI). Histological evaluations were performed every 3-4 months. Upon achieving complete remission (CR), we recommended maintenance treatments including LNG-IUD, cyclical oral contraceptives, or low-dose cyclic progestin until they began attempting conception. Regular follow-up was conducted for all patients. The chi-square method was utilized to compare oncological and fertility outcomes, while the Cox proportional hazards regression analysis helped identify risk factors for CR, recurrence, and pregnancy. Results Overall, 55 (90.2%) patients achieved CR, including 90.9% of AEH patients and 89.7% of EC patients. The median re-treatment time was 6 months (ranging from 3 to 12 months). The CR rate for GnRHa alone, GnRHa + LNG-IUD and GnRHa + AI were 80.0%, 91.7% and 93.3%, respectively. After a median follow-up period of 36 months (ranging from 3 to 96 months), 19 women (34.5%) experienced recurrence, 40.0% in AEH and 31.4% in EC patients, with the median recurrence time of 23 months (ranging from 6 to 77 months). Among the patients who achieved CR, 39 expressed a desire to conceive, 20 (51.3%) became pregnant, 11 (28.2%) had successfully deliveries, 1 (5.1%) was still pregnant, while 8 (20.5%) suffered miscarriages. Conclusion GnRHa-based fertility-sparing treatment exhibited promising oncological and reproductive outcomes for progestin-resistant patients. Future larger multi-institutional studies are necessary to confirm these findings. Keywords: Endometrial cancer, Atypical endometrial hyperplasia, Fertility-sparing re-treatment, Progestin resistant
Revegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in ...maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have extensive application prospects in environmental purification, biotechnology, medicine and cosmetics, food industry, and renewable energy. To identify the high biomass of microalgae present in nature, microalgae with the greatest biomass were screened from natural water samples through filtration, pre-culture, and plate scribing separation. Following identification via 18S rRNA sequencing as for the Desmodesmus sp., we constructed a neighbor-joining phylogenetic tree. The novel Desmodesmus sp. from the Tibetan Yamdrok Lake were identified through polyphasic taxonomy. Simultaneously, the sequence of the experimental samples and the target species were shown different following the identification and analysis of SNP and InDel loci. The light-absorbing properties of plateau Desmodesmus sp. have been investigated previously. The characteristic absorption peak of Desmodesmus sp. on the plateau was measured at 689 nm in the visible spectrum using full wavelength scanning with a UV-Vis spectrophotometer. For Desmodesmus sp. which is prone to settling in the process of amplification culture. By monitoring the change trend of total nitrogen, total phosphorus, pH and electrical conductivity in algae solution system, we determined that the logarithmic growth phase and the best transfer window of Desmodesmus sp. were at 15-20 days. This study can provide basic research methods for the study of microalgae in high altitude areas, and lay a foundation for the later study and application of microalgae.
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