Mucin-domain glycoproteins expressed on cancer cell surfaces play central roles in cell adhesion, cancer progression, stem cell renewal, and immune evasion. Despite abundant evidence that ...mucin-domain glycoproteins are critical to the pathobiology of head and neck squamous cell carcinoma (HNSCC), our knowledge of the composition of that mucinome is grossly incomplete. Here, we utilized a catalytically inactive point mutant of the enzyme StcE (StcEE447D) to capture mucin-domain glycoproteins in head and neck cancer cell line lysates followed by their characterization using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), in-gel digestion, nano-liquid chromatography–tandem mass spectrometry (nLC-MS/MS), and enrichment analyses. We demonstrate the feasibility of this workflow for the study of mucin-domain glycoproteins in HNSCC, identify a set of mucin-domain glycoproteins common to multiple HNSCC cell lines, and report a subset of mucin-domain glycoproteins that are uniquely expressed in HSC-3 cells, a cell line derived from a highly aggressive metastatic tongue squamous cell carcinoma. This effort represents the first attempt to identify mucin-domain glycoproteins in HNSCC in an untargeted, unbiased analysis, paving the way for a more comprehensive characterization of the mucinome components that mediate aggressive tumor cell phenotypes. Data associated with this study have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifier PXD029420.
The ALICE Zero Degree Calorimeters (ZDC) provide information about event geometry in heavy-ion collisions through the detection of spectator nucleons and allow to estimate the delivered luminosity. ...They are also very useful in p–A collisions, allowing an unbiased estimation of collision centrality. The Run 3 operating conditions will involve a tenfold increase in instantaneous luminosity in heavy-ion collisions, with event rates that, taking into account the different processes, could reach 5 MHz in the ZDCs. The challenges posed by this demanding environment lead to a redesign of the readout system and to the transition to a continuous acquisition. The new system is based on 12 bit, 1 Gsps FMC digitizers that will continuously sample the 26 ZDC channels. Triggering, pedestal estimation and luminosity measurements will be performed on FPGA directly connected to the front-end. The new readout system and the performances foreseen in Run 3 are presented.
Mini-EUSO is part of the JEM-EUSO program and operates on board the International Space Station (ISS). It is a UV-telescope with single-photon counting capability looking at nighttime downwards to ...the Earth through a nadir-facing UV-transparent window. As part of the pre-flight tests, the Mini-EUSO engineering model, a telescope with 1/9 of the original focal surface and a lens of 2.5 cm diameter, has been built and tested. Tests of the Mini-EUSO engineering model have been made in laboratory and in open-sky conditions. Laboratory tests have been performed at the TurLab facility, located at the Physics Department of the University of Turin, equipped with a rotating tank containing different types of materials and light sources. In this way, the configuration for the observation of the Earth from space was emulated, including the Mini-EUSO trigger schemes. In addition to the qualification and calibration tests, the Mini-EUSO engineering model has also been used to evaluate the possibility of using a JEM-EUSO-type detector for applications such as observation of space debris. Furthermore, observations in open-sky conditions allowed the studies of natural light sources such as stars, meteors, planets, and artificial light sources such as airplanes, satellites reflecting the sunlight, and city lights. Most of these targets could be detected also with Mini-EUSO. In this paper, the tests in laboratory and in open-sky conditions are reported, as well as the obtained results. In addition, the contribution that such tests provided to foresee and improve the performance of Mini-EUSO on board the ISS is discussed.
The EUSO@TurLab project aims at performing experiments to reproduce Earth UV emissions as seen from a low Earth orbit by the planned missions of the JEM-EUSO program. It makes use of the TurLab ...facility, which is a laboratory, equipped with a 5 m diameter and 1 m depth rotating tank, located at the Physics Department of the University of Turin. All the experiments are designed and performed based on simulations of the expected response of the detectors to be flown in space. In April 2016 the TUS detector and more recently in October 2019 the Mini-EUSO experiment, both part of the JEM-EUSO program, have been placed in orbit to map the UV Earth emissions. It is, therefore, now possible to compare the replicas performed at TurLab with the actual images detected in space to understand the level of fidelity in terms of reproduction of the expected signals. We show that the laboratory tests reproduce at the order of magnitude level the measurements from space in terms of spatial extension and time duration of the emitted UV light, as well as the intensity in terms of expected counts per pixel per unit time when atmospheric transient events, diffuse nightlow background light, and artificial light sources are considered. Therefore, TurLab is found to be a very useful facility for testing the acquisition logic of the detectors of the present and future missions of the JEM-EUSO program and beyond in order to reproduce atmospheric signals in the laboratory.
The experiment BESIII, running at the accelerator BEPCII in Beijing (P.R.C.), is going to be updated with the replacement of the Inner Drift Chamber with a Cylindrical triple-GEM Inner Tracker ...(CGEM-IT). In the R&D stage, two standalone C++ codes were implemented: GTS (Garfield-based Triple-GEM Simulator), for digitization and tuning of simulated data to the experimental ones, and GRAAL (GEM Reconstruction And Analysis Library), for the reconstruction and analysis of the experimental events collected in testbeams. GTS simulates the triple-GEM response to the particle passage, treating each stage separately: ionization, GEM properties, gas mixture, magnetic field and finally the induction of the signal on the anode. The necessary information was extracted by GARFIELD++ simulations, parametrized and used as input in GTS. This speeds up the simulation, since GTS performs only samplings instead of the full digitization chain. The simulated events were reconstructed with the same procedure used for experimental data and tuning factors were evaluated to obtain a satisfactory match. GRAAL is used in the analysis of the testbeam experimental data. It provides several levels of reconstruction: from the cluster formation, gathering contiguous firing strips, to the spatial position and the signal time reconstruciton. Two algorithms are used: the charge centroid and the micro-TPC, which exploit the charge deposition on the strips and the time information. Also a merging of the two algorithms is available to efficiently weight the two outcomes and obtain the best estimate of the spatial coordinate. Moreover, GRAAL performs tracking and alignment. Both codes are going to be made available also for other MPGDs simulation and reconstruction.
The EUSO@TurLab project is an on-going activity aimed to reproduce atmospheric and luminous conditions that JEM-EUSO will encounter on its orbits around the Earth. The use of the TurLab facility, ...part of the Department of Physics of the University of Torino, allows the simulation of different surface conditions in a very dark and rotating environment in order to test the response of JEM-EUSO's sensors and sensitivity. The experimental setup currently in operation has been used to check the potential of the TurLab facility for the above purposes, and the acquired data will be used to test the concept of JEM-EUSO's trigger system.
Abstract
Mini-EUSO is a telescope observing the Earth in the ultraviolet band from the International Space Station. It is a part of the JEM-EUSO program, paving the way to future larger missions, ...such as K-EUSO and POEMMA, devoted primarily to the observation of ultrahigh-energy cosmic rays from space. Mini-EUSO is capable of observing extensive air showers generated by ultrahigh-energy cosmic rays with an energy above 10
21
eV and to detect artificial showers generated with lasers from the ground. Other main scientific objectives of the mission are the search for nuclearites and strange quark matter, the study of atmospheric phenomena such as transient luminous events, meteors, and meteoroids, the observation of sea bioluminescence and of artificial satellites and man-made space debris. Mini-EUSO will map the nighttime Earth in the UV range (290–430 nm), with a spatial resolution of about 6.3 km and a temporal resolution of 2.5
μ
s, through a nadir-facing UV-transparent window in the Russian Zvezda module. The instrument, launched on 2019 August 22, from the Baikonur Cosmodrome, is based on an optical system employing two Fresnel lenses and a focal surface composed of 36 multianode photomultiplier tubes, 64 channels each, for a total of 2304 channels with single-photon counting sensitivity and an overall field of view of 44°. Mini-EUSO also contains two ancillary cameras to complement measurements in the near-infrared and visible ranges. In this paper, we describe the detector and present the various phenomena observed in the first months of operations.
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