Abnormalities involving chromosome 13 have been reported as the only cytogenetic change in B-cell chronic lymphocytic leukemia (BCLL). Deletions are the most common cytogenetic abnormality and always ...involve 13q14, but when translocations are seen, the consistent breakpoint is always in 13q14. It is now established that deletions, distal to the RB1 gene in 13q14, are invariably associated with these translocations. We have recently described the smallest such deletion from a series of rearrangements from these tumors isolated in somatic cell hybrids, which spans approximately 1 Mb. In this report, we present the results of a series of a chromosome walking experiments using YACs and have been able to span this small deletion, which must contain the gene that is frequently deleted in BCLL. Four probes from 13q14 (RB1-mgg15-D13S25-D13S31) were used to isolate corresponding YACs for each of the markers. The chromosomal location of these YACs was verified using FISH, which also demonstrated their nonchimeric nature. Vectorette end rescue was then used to demonstrate the overlap of the YACs and to isolate new clones to complete the contig. The extremes of the contig were shown to cross the chromosome 13 translocation breakpoints isolated in somatic cell hybrids that carry the derivatives of chromosome 13 involved in the smallest BCLL deletion. This YAC contig covers the entire deletion and will prove a valuable resource to begin isolating genes from this region. In addition, we have isolated YACs corresponding to the RB1 locus, which extends the contig over a 3.8-cM distance on the chromosome.
A deletion hybrid breakpoint map of the chromosomal region 13q14-q21 has been constructed using 19 DNA markers and 13 cell lines with breakpoints in this chromosomal region. The cell lines define 10 ...distinct intervals in this region, which spans approximately 20 Mb. The markers include 6 RFLP markers, 11 microsatellites that provisionally had been mapped to the region 13q14-21, and 2 new polymorphic CA-repeats that were developed from an EMBL3 library of cell line ICD, containing 13pter-q14.3. The following order of markers was established: CEN-D13S320-(D13S118, D13S153)-RB1-D13S319-D13S25-(D13S31, D13S59, D13S133, D13S137)-D13S163-D13S119-(D13S26, D13S55)-(D13S131, D13S134, D13S135, D13S144, D13S152)-TEL.
Titre uniforme : Le jupon du Portugal
Titre uniforme : Clases de cha-cha-cha
Titre uniforme : Que serà, serà
Titre uniforme : Le Jupon du Portugal
Titre uniforme : Le Facteur de Santa Cruz
Comprend : ...LES AMES FIERES : fox / NEWMAN - EN FLANANT SUR LES CHAMPS-ELYSEES : fox / GIACOMAZZI - CLASSE DE CHA CHA CHA / R. MARQUEZ - TRAHISON : tango / G. GOMEZ - MANTILLA ANDALUZA : paso-doble / ALCARA - AIE, QU'ELLE EST JOLIE : boléro / R. EMMERECHTS et B. ANSERMINI - LE COR DES ALPES : valse / W. CARSTEN - LE FACTEUR DE SANTA CRUZ : guaracha / F. BARCELLINI - MAIN DANS LA MAIN : slow / COWELL - BOLERO POUR TOI / R. LECUSSANT - LE JUPON DU PORTUGAL : mambo / DURSO et Mitchell KAHN - MIGUEL ESTEBAN : paso-doble / L. ARAQUE - SANS RANCUNE : tango / M. HERRAND - QUE SERA SERA : valse / J. LIVINGSTON et R. EVANS
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Uniform title: Clases of a cha-cha-cha
Titre uniforme : Le jupon du Portugal
Titre uniforme : Clases de cha-cha-cha
Titre uniforme : Que serà, serà
Titre uniforme : Le Jupon du Portugal
Titre uniforme : Le Facteur de Santa Cruz
BnF-Partenariats, Collection sonore - Believe
Contient une table des matières
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