Alzheimer's disease (AD) is a diffused neurodegenerative disorder affecting people in advanced age causing loss of memory and dementia. Nowadays, diagnosis and treatment of AD are still challenging ...due to the lack of diagnostic systems that allow for an early and reliable diagnosis and therapy monitoring. Moreover, conventional strategies for AD diagnosis are based on brain imaging techniques that are invasive and expensive for early and massive screening. Phage display approach, using engineered phage probe for direct amyloid‐β (Aβ)‐autoantibodies detection, overcome these limitations leading to the possibility of safe and low‐cost screening. Moreover, the combination with silicon technology further improves the easiness of diagnosis due to the portability of devices and the integration of sensitive transduction signals. In this work, an innovative silicon‐based sensing technology is reported detecting Aβ‐autoantibodies, specifically Immunoglobulin G (IgG), in human sera by engineered M13‐phage probes (ADPP). The strategy hinges on a bio‐surface that is integrated on top of a silicon biosensor. Thanks to phages probes exposing Aβ‐mimic peptides, this chip can capture and reveal Aβ‐autoantibodies, discriminating between healthy and AD conditions. The surface chemistry is morphologically and chemically characterized and the phage‐based biosensor ability to recognise Aβ‐autoantibodies is proved by transduction with enzyme‐linked anti‐M13 antibodies.
The proposed sensing surface relies on the combination between phage display and silicon technology to directly detect amyloid‐β (Aβ)‐autoantibodies from Alzheimer's disease (AD) affected human sera. Thanks to the integration in a miniaturized silicon chip, the technology opens toward a new frontier of low‐cost, non‐invasive, and decentralized AD diagnosis in a PoC format.
Achieving good adherence to self-injected treatments for multiple sclerosis can be difficult. Injection devices may help to overcome some of the injection-related barriers to adherence that can be ...experienced by patients. We sought to assess short-term adherence to, and tolerability of, interferon (IFN) β-1a administered via electronic autoinjection device in patients with relapsing-remitting multiple sclerosis (RRMS).
BRIDGE (RebiSmart to self-inject Rebif serum-free formulation in a multidose cartridge) was a 12-week, multicentre, open-label, single-arm, observational, Phase IV study in which patients self-administered IFN β-1a (titrated to 44 μg), subcutaneously (sc), three times weekly, via electronic autoinjection device. Patients were assessed at baseline and 4-weekly intervals to Week 12 or early termination (ET) for: physical examinations; diary card completion (baseline, Weeks 4, 8 only); neurological examinations (baseline, Week 12/ET only); MS Treatment Concern Questionnaire (MSTCQ; Weeks 4, 8, 12 only); Convenience Questionnaire (Week 12 only); Hospital Anxiety and Depression Scale (HADS); and Paced Auditory Serial Addition Task (PASAT; baseline only). Adherence was defined as administration of ≥ 80% of scheduled injections, recorded by the autoinjection device.
Overall, 88.2% (105/119; intent-to-treat population) of patients were adherent; 67.2% (80/119) administered all scheduled injections. Medical reasons accounted for 35.6% (31/87) of missed injections, forgetfulness for 20.6% (18/87). Adherence did not correlate with baseline Expanded Disability Status Scale (P = 0.821) or PASAT (P = 0.952) scores, or pre-study therapy (P = 0.303). No significant changes (baseline-Week 12) in mean HADS depression (P = 0.482) or anxiety (P = 0.156) scores were observed. 'Overall convenience' was the most important reported benefit of the autoinjection device. Device features associated with handling and ease of use were highly rated. Mean MSTCQ scores for 'flu-like' symptoms (P = 0.022) and global side effects (P = 0.002) significantly improved from Week 4-12. Mean MSTCQ scores for pain at injection site and injection pain increased from Week 4-12 (P < 0.001). Adverse events were mild/moderate. No new safety signals were identified.
Convenience and ease of use of the autoinjection device may improve adherence and, therefore, outcomes, in patients with RRMS receiving sc IFN β-1a.
EU Clinical Trials Register (EU-CTR; http://www.clinicaltrialsregister.eu): 2009-013333-24.
The EU Member States are being involved to develop long-term strategies and to promote investments aimed at improving the energy efficiency of the building stock, at increasing the use of Renewable ...Energy Sources (RES) and at growing the number of Nearly Zero-Energy Buildings (NZEBs). The aim of this article is to investigate energy and economic implications related to the exploitation of RES in the transformation of an Italian social housing building-type into a NZEB. The research is based on a detailed energy audit procedure that includes cost-optimal assessment and compliance check with the legal requirements. A parametric analysis was performed to find out the technical building system configurations that verify the minimum share of RES established by the Italian regulations, and at the same time to assess global cost and payback period. The intersection between legal compliancy and cost-effectiveness narrows the field of applicable RES technologies that are limited to electric heat pump for heating and cooling coupled with PV system, and low size solar collectors coupled with low temperature generator for domestic hot water. Improvements in the energy policy are necessary to guarantee the best trade-off between RES exploitation, energy efficiency and costs, as to preserve market equilibrium.
•The economic implications of the minimum RES share set by regulations are assessed.•The analysis is performed on a social housing building transformed into NZEB.•Cost-optimal technical building systems using RES are identified.•Cost-optimal energy efficiency measures do not comply with legal EP requirements.•The heat pump coupled with the photovoltaic system is a cost-effective solution.
This paper presents an effective and efficient method to fabricate novel fiber optic sensing probes. The new, simple, and low cost approach is based on a 3-D photonic crystal dielectric structure ...directly deposited on the tip of a multimode optical fiber through the self-assembly of colloidal crystals (CCs) via a vertical deposition technique. Here, the CC is made of polystyrene nanospheres with 200 nm diameter, and the optical fiber is a UV-vis fiber with a core diameter of 200 μm. The obtained fiber probes exhibit a resonant peak at 480 nm and an amplitude enhancement of 3.7 with respect to the bare fiber; these results are highly repeatable. A numerical tool based on a finite element method analysis has been developed to study and analyze the 3-D subwavelength structures. Numerical results are in good agreement with the observed experimental spectra. Moreover, refractive index measurements have been carried out, revealing a sensitivity of up to 445 nm/RIU in the 1.33-1.36 values range. The achieved performances, which have been obtained by using very small active areas and an easy and reliable fabrication procedure, demonstrate the future perspectives of these fiber-optic probes for chemical and biological sensing applications.
The Spirorchiidae is a family of blood flukes parasitizing turtles. Spirorchiids may cause a wide range of inflammatory reactions in the vascular system of their host being frequently implicated with ...stranding and death of sea turtles worldwide. Recent studies revealed the presence of two spirorchiid species in the Mediterranean basin. Our study presents comparative epidemiological data of spirorchiid infections in loggerhead turtles (Caretta caretta) stranded during an eight-year period from Adriatic and Tyrrhenian Seas, and the first report of Neospirorchis Neogen-11 in a green turtle (Chelonia mydas).
We screened a total of 319 carcasses of loggerhead turtles stranded from January 2011 to December 2018 along the Tyrrhenian coast (n = 111) and the north-western Adriatic coast (n = 208) of Italy using traditional (copromicroscopy and histopathology) and molecular assays. Three green turtles from the Tyrrhenian coast were also included in the study.
A total of 56 (17.5%) loggerhead turtles and one green turtle (33.3%) were found to be infected with spirorchiid flukes. Amplification, sequencing of the ITS2 region of the ribosomal RNA gene cluster and BLAST analysis confirmed the presence of Hapalotrema mistroides and Neospirorchis Neogen-11 in 51 (16.0%) and 24 (7.5%) loggerhead turtles, respectively, and Neospirorchis Neogen-11 in an infected green turtle. Differences in prevalence of infection between the two sampling areas were found.
The risk of spirorchiid infection in the Tyrrhenian Sea is lower than in the Adriatic Sea and in general the risk of infection in the Mediterranean is lower than in other geographical locations. Differences in the prevalence of infection between the two sampling areas were related to the differences of regional habitats supporting different abundance of spirorchiid intermediate hosts. A systematic monitoring to evaluate the progress of the infection is recommended, as well as studies on the occurrence and distribution of spirorchiid species from other Mediterranean areas.
Objective
This study was undertaken to assess the impact of immunosuppressive and immunomodulatory therapies on the severity of coronavirus disease 2019 (COVID‐19) in people with multiple sclerosis ...(PwMS).
Methods
We retrospectively collected data of PwMS with suspected or confirmed COVID‐19. All the patients had complete follow‐up to death or recovery. Severe COVID‐19 was defined by a 3‐level variable: mild disease not requiring hospitalization versus pneumonia or hospitalization versus intensive care unit (ICU) admission or death. We evaluated baseline characteristics and MS therapies associated with severe COVID‐19 by multivariate and propensity score (PS)‐weighted ordinal logistic models. Sensitivity analyses were run to confirm the results.
Results
Of 844 PwMS with suspected (n = 565) or confirmed (n = 279) COVID‐19, 13 (1.54%) died; 11 of them were in a progressive MS phase, and 8 were without any therapy. Thirty‐eight (4.5%) were admitted to an ICU; 99 (11.7%) had radiologically documented pneumonia; 96 (11.4%) were hospitalized.
After adjusting for region, age, sex, progressive MS course, Expanded Disability Status Scale, disease duration, body mass index, comorbidities, and recent methylprednisolone use, therapy with an anti‐CD20 agent (ocrelizumab or rituximab) was significantly associated (odds ratio OR = 2.37, 95% confidence interval CI = 1.18–4.74, p = 0.015) with increased risk of severe COVID‐19. Recent use (<1 month) of methylprednisolone was also associated with a worse outcome (OR = 5.24, 95% CI = 2.20–12.53, p = 0.001). Results were confirmed by the PS‐weighted analysis and by all the sensitivity analyses.
Interpretation
This study showed an acceptable level of safety of therapies with a broad array of mechanisms of action. However, some specific elements of risk emerged. These will need to be considered while the COVID‐19 pandemic persists. ANN NEUROL 2021;89:780–789
We investigated Legionella and Pseudomonas contamination of hot water in a cross-sectional multicentric survey in Italy. Chemical parameters (hardness, free chlorine, and trace elements) were ...determined. Legionella spp. were detected in 33 (22.6%) and Pseudomonas spp. in 56 (38.4%) of 146 samples. Some factors associated with Legionella contamination were heater type, tank distance and capacity, water plant age, and mineral content. Pseudomonas presence was influenced by water source, hardness, free chlorine, and temperature. Legionella contamination was associated with a centralized heater, distance from the heater point >10 m, and a water plant >10 years old. Furthermore, zinc levels of <100 microg/L and copper levels of >50 microg/L appeared to be protective against Legionella colonization corrected. Legionella species and serogroups were differently distributed according to heater type, water temperature, and free chlorine, suggesting that Legionella strains may have a different sensibility and resistance to environmental factors and different ecologic niches.
Many studies investigated the association between air pollution and Covid-19 severity but the only study focusing on patients with Multiple Sclerosis (MS) exclusively evaluated exposure to PM2.5. We ...aim to study, in a sample of MS patients, the impact of long-term exposure to PM2.5, PM10 and NO2 on Covid-19 severity, described as occurrence of pneumonia.
A 1:2 ratio case-control study was designed, differentiating cases and controls based on Covid-19 pneumonia. Associations between pollutants and outcome were studied using logistic regression. Weighted quantile sum (WQS) logistic regression was used to identify the individual contribution of each pollutant within the mixture; Least Absolute Shrinkage and Selection Operator (LASSO) penalized regression was performed to confirm the variable selection from WQS. All the analyses were adjusted for confounders selected a priori.
Of the 615 eligible patients, 491 patients provided detailed place of exposure and were included in the principal analysis. Higher concentrations of air pollutants were associated with increased odds of developing Covid-19 pneumonia (PM2.5: 3rd vs 1st tercile OR(95% CI)=2.26(1.29;3.96); PM10: 3rd vs 1st tercile OR(95% CI)=2.12(1.22;3.68); NO2: 3rd vs 1st tercile OR(95% CI)=2.12(1.21;3.69)). Pollutants were highly correlated with each other; WQS index was associated to an increased risk of pneumonia (β=0.44; p-value=0.004) and the main contributors to this association were NO2 (41%) and PM2.5 (34%). Consistently, Lasso method selected PM2.5 and NO2.
Higher long-term exposure to PM2.5, PM10 and NO2 increased the odds of Covid-19 pneumonia among MS patients and the most dangerous pollutants were NO2 and PM2.5.
The tetrabutylammonium salt of 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TPPS) is soluble in dichloromethane, and the general properties of this compound have been investigated as function of ...various added acids HX (X = Cl, Br, I, CF3COO, CF3SO3, TFPB) through UV−vis absorption spectroscopy, steady state fluorescence emission, and resonance light-scattering (RLS) techniques. Upon addition of HX, the initial monomeric free base TPPS is readily converted in an aggregated species, whose spectroscopic features are independent of the nature of the counteranion X. All the spectroscopic evidence suggest a J-type arrangement of chromophores in this aggregate, involving strong hydrogen bonds, electrostatic, and dispersive interactions. In the specific case of chloride and bromide, in the presence of a TBAX excess, the addition of the corresponding acid leads to a monomeric ion-pair between the TBA cations and the diacid TPPS, whose central core is strongly interacting with the halide. On further increasing the acid concentration in these latter solutions, fully protonated species are formed that eventually start to aggregate.
The microbial removal efficacy of membrane bioreactor (MBR) technology in a full-scale municipal wastewater treatment plant was evaluated. Total and faecal coliforms, thermo-tolerant coliforms,
...Escherichia coli, enterococci, somatic coliphages, F-RNA specific bacteriophages and bacteriophages infecting
Bacteroides fragilis (BFRPH) were used as bacterial and viral indicators. Reclamation and reuse of MBR effluent were considered.
The average Log
10 reductions of the bacterial indicators range from 6 Log
10 for enterococci to 7 Log
10 for
E. coli and faecal coliforms. The average removal of FRNA coliphages was similar to that of enterococci. Complete removal of BFRPH was observed. Log
10 reductions of somatic coliphages were significantly lower than those of all other indicators.
Somatic coliphages appear to be better indicators of the microbial removal efficiency of the MBR system than faecal bacterial indicators.
The quality of the MBR permeate conforms largely with the microbiological standards for urban (e.g. street cleaning, vehicle washing) and agricultural reuse.
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