Patients with liver cirrhosis exhibit early onset of gluconeogenesis after short‐term fasting. This accelerated metabolic reaction to starvation may underlie their increased protein requirements and ...muscle depletion. A randomized controlled trial was conducted to test the hypothesis that provision of a late‐evening nutritional supplement over a 12‐month period would improve body protein stores in patients with cirrhosis. A total of 103 patients (68 male, 35 female; median age 51, range 28–74; Child‐Pugh grading: 52A, 31B, 20C) were randomized to receive either daytime (between 0900 and 1900 hours) or nighttime (between 2100 and 0700 hours) supplementary nutrition (710 kcal/day). Primary etiology of liver disease was chronic viral hepatitis (67), alcohol (15), cholestatic (6), and other (15). Total body protein (TBP) was measured by neutron activation analysis at baseline, 3, 6, and 12 months. Total daily energy and protein intakes were assessed at baseline and at 3 months by comprehensive dietary recall. As a percentage of values predicted when well, TBP at baseline was similar for the daytime (85 ± 2standard error of the mean%) and nighttime (84 ± 2%) groups. For the nighttime group, significant increases in TBP were measured at 3 (0.38 ± 0.10 kg, P = 0.0004), 6 (0.48 ± 0.13 kg, P = 0.0007), and 12 months (0.53 ± 0.17 kg, P = 0.003) compared to baseline. For the daytime group, no significant changes in TBP were seen. Daily energy and protein intakes at 3 months were higher than at baseline in both groups (P < 0.0001), and these changes did not differ between the groups. Conclusion: Provision of a nighttime feed to patients with cirrhosis results in body protein accretion equivalent to about 2 kg of lean tissue sustained over 12 months. This improved nutritional status may have important implications for the clinical course of these patients. (HEPATOLOGY 2008.)
Although the recruitment of fibroblasts to areas of injury is critical for wound healing, their subsequent apoptosis is necessary in order to prevent excessive scarring. Fibroproliferative diseases, ...such as pulmonary fibrosis, are often characterized by fibroblast resistance to apoptosis, but the mechanism(s) for this resistance remains elusive. Here, we employed a murine model of pulmonary fibrosis and cells from patients with idiopathic pulmonary fibrosis (IPF) to explore epigenetic mechanisms that may be responsible for the decreased expression of Fas, a cell surface death receptor whose expression has been observed to be decreased in pulmonary fibrosis. Murine pulmonary fibrosis was elicited by intratracheal injection of bleomycin. Fibroblasts cultured from bleomycin-treated mice exhibited decreased Fas expression and resistance to Fas-mediated apoptosis compared with cells from saline-treated control mice. Although there were no differences in DNA methylation, the Fas promoter in fibroblasts from bleomycin-treated mice exhibited decreased histone acetylation and increased histone 3 lysine 9 trimethylation (H3K9Me3). This was associated with increased histone deacetylase (HDAC)-2 and HDAC4 expression. Treatment with HDAC inhibitors increased Fas expression and restored susceptibility to Fas-mediated apoptosis. Fibroblasts from patients with IPF likewise exhibited decreased histone acetylation and increased H3K9Me3 at the Fas promoter and increased their expression of Fas in the presence of an HDAC inhibitor. These findings demonstrate the critical role of histone modifications in the development of fibroblast resistance to apoptosis in both a murine model and in patients with pulmonary fibrosis and suggest novel approaches to therapy for progressive fibroproliferative disorders.
Aims: To develop a quantitative PCR assay for sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (Map) in a range of dairy products.
Methods and Results: TaqMan® assays ...were designed to target the IS900 and f57 genetic elements of Map. Both real‐time PCR assays were integrated with the Adiapure® Map DNA extraction kit and assessed separately for the detection/quantification of Map in spiked milk, Cheddar cheese and milk powder. Assays were validated against Cheddar cheese samples containing known concentrations of Map. The IS900 qPCR assay was significantly more sensitive than the assay based on the f57 primer/probe. At a threshold cycle value of 38, limits of detection (LOD) for the IS900 qPCR assay were 0·6 CFU ml−1, 2·8 CFU g−1 and 30 CFU g−1 for artificially contaminated pasteurized milk, whole milk powder and Cheddar cheese, respectively. The respective LOD’s for the f57 assay were 6·2 CFU ml−1, 26·7 CFU g−1 and 316 CFU g−1.
Conclusion: The integrated Adiapure® extraction – IS900 real time assay described is a sensitive, quantitative method for the detection of Map in dairy products. This is the first study to consider qPCR as a quantitative estimation of Map‐DNA in cheese and whole milk powder.
Significance and Impact of the Study: The assay developed allows sensitive detection and quantification of Map DNA in a range of dairy products which is valuable for the screening and surveillance of this potential zoonotic organism.
Incorporating plantain with perennial ryegrass and white clover (RGWC) can improve the quality and quantity of grazing pastures; however, the sowing rate could affect the persistence of plantain, ...pasture yield, and nutritive value in different seasons. The objective of this study was to evaluate the effect of increasing sowing rates of plantain when established with RGWC on the dry matter (DM) yield, botanical composition, nutritive value, and bioactive compounds of the pasture over the first two years after sowing; and to determine the relationship between plantain content and nutritive characteristics in different seasons. The pasture treatments were RGWC, RGWC + low plantain rate (PLL), RGWC + medium plantain rate (PLM), and RGWC + high plantain rate (PLH). The results showed that annual DM yield was similar between treatments. The average plantain content (including leaves and reproductive stem) was 32, 44, and 48% in PLL, PLM, and PLH, respectively. The plantain composition increased in the first 15 months, then declined rapidly to about 20–30% at day 705 after sowing. Compared with RGWC, the plantain-RGWC pastures (PLL, PLM, PLH) had a higher content of organic matter digestibility (OMD), ash, starch, non-structural carbohydrates (NSC), P, S, Ca, Mg, Na, Cl, Zn, B, Co, aucubin, acteoside, and catalpol, while they contained a lower composition of DM%, acid detergent fibre (ADF), neutral detergent fibre (NDF), crude fat (CF), Fe, and Mn. These differences were linearly associated with the content of plantain leaves in the pasture and were higher in summer and autumn than in spring. In conclusion, incorporating plantain into the RGWC pasture can improve herbage nutritive quality, thus potentially increasing farm productivity and environmental benefits. However, further work is required to investigate management interventions to sustain plantain content beyond two years from sowing.
The demand for dairy products is ever increasing across the world. The livestock sector is a significant source of greenhouse gas (GHG) emissions globally. The availability of high-quality pasture is ...a key requirement to increase the productivity of dairy cows as well as manage enteric methane emissions. Warm-season perennial grasses are the dominant forages in tropical and subtropical regions, and thus exploring their nutritive characteristics is imperative in the effort to improve dairy productivity. Therefore, we have collated a database containing a total of 4750 records, with 1277 measurements of nutritive values representing 56 tropical pasture species and hybrid cultivars grown in 26 different locations in 16 countries; this was done in order to compare the nutritive values and GHG production across different forage species, climatic zones, and defoliation management regimes. Average edaphoclimatic (with minimum and maximum values) conditions for tropical pasture species growing environments were characterized as 22.5 °C temperature (range 17.5–29.30 °C), 1253.9 mm rainfall (range 104.5–3390.0 mm), 582.6 m elevation (range 15–2393 m), and a soil pH of 5.6 (range 4.6–7.0). The data revealed spatial variability in nutritive metrics across bioclimatic zones and between and within species. The ranges of these nutrients were as follows: neutral detergent fibre (NDF) 50.9–79.8%, acid detergent fibre (ADF) 24.7–57.4%, crude protein (CP) 2.1–21.1%, dry matter (DM) digestibility 30.2–70.1%, metabolisable energy (ME)3.4–9.7 MJ kg−1 DM, with methane (CH4) production at 132.9–133.3 g animal−1 day−1. The arid/dry zone recorded the highest DM yield, with decreased CP and high fibre components and minerals. Furthermore, the data revealed that climate, defoliation frequency and intensity, in addition to their interactions, have a significant effect on tropical pasture nutritive values and CH4 production. Overall, hybrid and newer tropical cultivars performed well across different climates, with small variations in herbage quality. The current study revealed important factors that affect pasture nutritive values and CH4 emissions, with the potential for improving tropical forage through the selection and management of pasture species.
Aims
Persistence of Mycobacterium bovis was investigated in UK raw milk cheeses.
Methods and Results
Replicating traditional cheese production methods under stringent CL3 containment conditions, ...Cheddar and Caerphilly cheeses were produced with Myco. bovis inoculated raw milk. High‐inoculum investigations used three Myco. bovis genotypes; later low‐inoculum investigations used only Myco. bovis AF2122/97. High‐inoculum Cheddar (n = 9) and Caerphilly (n = 9) were matured for a minimum of 12 and 4 months respectively; maturation of low‐inoculum Cheddar (n = 3) and Caerphilly (n = 3) was up to 11 weeks. Survival of Myco. bovis was monitored by enumeration at different points throughout cheese manufacture and ripening. D values were calculated as follows: 57 and 59 days in high‐inoculum Cheddar and Caerphilly, respectively, and 41 and 24 days in low‐inoculum Cheddar and Caerphilly respectively.
Conclusions
Mycobacterium bovis is concentrated in cheese curd and a proportion lost with the whey. Reduction in viability during manufacturing is limited, while significant Myco. bovis inactivation occurs during maturation. Inactivation was improved, during Caerphilly ripening, when acid development was enhanced by increasing the proportion of starter culture.
Significance and Impact of the Study
Mycobacterium bovis inactivation data obtained could be used to inform assessment of the risk posed to consumers by raw milk dairy products.
Exploring improved tropical forages is considered to be an important approach in delivering quality and consistent feed options for dairy cattle in tropical and subtropical regions. The present study ...aimed to study the suitability of three improved tropical grasses, Chloris gayana ‘Rhodes grass cv. Reclaimer’ (RR), Megathyrsus maximus ‘Gatton Panic’ (GP), and Brachiaria ruziziensis x B. decumbens x B. brizantha ‘Brachiaria Mulato II’ (BM) evaluating their carbon assimilation, canopy structure, herbage plant–part accumulation and quality parameters under irrigated conditions. An experiment was conducted at Gatton Research Dairy (27°54′ S, 152°33′ E, 89 m asl) Queensland, Australia, which has a predominantly subtropical climate. Photosynthesis biochemistry, canopy structure, herbage accumulation, plant part composition, and nutritive value were evaluated. Photosynthesis biochemistry differed between pasture species. Efficiency of CO2 assimilation was highest for GP and quantum efficiency was highest for BM. Pasture canopy structure was significantly affected by an interaction between pasture species and harvest. Forage biomass accumulation was highest in GP, while BM produced more leaf and less stem compared to both GP and RR. A greater leafy stratum and lower stemmy stratum depth were observed in the vertical sward structure of BM. Brachiaria Mulato II showed greater carbon partitioning to leaves, leaf: stem ratio, canopy, and leaf bulk density. It also demonstrated greater nutritive value (Total digestible nutrients (TDN), acid detergent fibre (ADF), neutral detergent fibre (NDF), neutral detergent insoluble protein (NDICP), Starch, nonfibre carbohydrates (NFC), metabolisable energy (ME), mineral profile (Mg, P, K, Fe, Zn) and dietary cation–anion difference (DCAD) for leaf, stem, and the whole plant. Greater quantum efficiency, leaf accumulation, and nutritive value of BM observed in the present study suggest BM as an attractive forage option for dairying that warrants further research in pasture-based systems in tropical and subtropical climates.
PDF
