Za očuvanje planeta Zemlje, a posebno u suočavanju s globalnim klimatskim, ekološkim i socijalnim promjenama, potrebno je ujedinjenje svih ljudskih potencijala. U tom smislu se u radu razmatra značaj ...Europskog zelenog plana i njegove najvažnije mjere - unaprjeđenje učinkovitog iskorištavanja resursa prelaskom na čisto kružno gospodarstvo. Navedena su načela sustava kružnog gospodarstva, poboljšanja, usavršavanja i osuvremenjivanja smjerova razvitka za što bolju i učinkovitiju implementaciju u gospodarstvo i općenito u društvo. Prikazana je situacija gospodarenja tekstilnim otpadom i primjena kružnog gospodarstva u tekstilnoj industriji u Republici Hrvatskoj, kao i u Europskoj uniji. Dodatno su predstavljene mogućnosti inženjerskog doprinosa u rješavanju problematike zaštite okoliša i održivosti tekstilnih tehnologija, posebno kroz učinkovito korištenje resursa - izborom sirovina i različitih visokoučinkovitih postupaka oplemenjivanja tekstila.
Analizirane su mogućnost primjene i optimizacija procesa kompresije i ekspanzije u tekstilnoj industriji, kao što su predenje, bojadisanje, kemijsko čišćenje, teksturiranje, te hlađenje procesne vode ...i pneumatika odnosno pneumatsko upravljanje. U svakoj tekstilnoj tvornici optimizacija troškova uključuje kontrolu gubitaka kako vodene pare, tako i procesne vode, kondenzata i komprimiranog zraka. Tekstilna tvornica često proizvodi više komprimiranog zraka nego je to potrebno kako bi se osigurali dovoljno visoki tlakovi za rad potrošačkih uređaja. Pri tome valja naglasiti kako je optimizacija sustava s komprimiranim zrakom od presudne važnosti jer svaki potrošački uređaj zahtijeva specifičnu vrijednost tlaka zraka. To se postiže kalibriranjem izlaznog tlaka zraka u uređajima za generiranje komprimiranog zraka i osiguravanjem optimalnog tlaka zraka za svaki pojedini potrošački uređaj.
U ovom radu analizira se pojam otvorene znanosti i uloga Sveučilišne knjižnice Rijeka u kontekstu preduvjeta implementacije otvorene znanosti u praksi, podizanja svijesti te provođenja znanosti na ...otvoren i suradnički način.
Podržavajući napredak znanosti i širenje znanja u korist društva, temeljeći svoj razvoj i usmjerenje na relevantnim nacionalnim i dokumentima Europske Unije, te čvrsto podupirući stav da znanstvene informacije i istraživački podaci koji su proizvod znanstvenih aktivnosti financiranih javnim sredstvima moraju imati otvoreni pristup, Sveučilište u Rijeci je u rujnu 2021. godine usvojilo Politiku otvorene znanosti Sveučilišta u Rijeci. Kako bi se aktivno uključila u provođenje Politike otvorene znanosti, Sveučilišna knjižnica Rijeka osnovala je Centar za otvorenu znanost i upravljanje znanstvenim informacijama. Centar za otvorenu znanost kontinuirano radi na podizanju razine svijesti i znanja dionika znanstvene djelatnosti Sveučilišta u Rijeci o značaju otvorenih znanstvenih informacija i istraživačkih podataka kao ključnom ishodu istraživanja.
Cilj ovog članka je na primjeru studije slučaja, Sveučilišne knjižnice Rijeka, prikazati ulogu visokoškolske knjižnice kao važnog dionika u pružanju potpore otvaranju cjelokupnog istraživačkog procesa i provođenju praksi otvorene znanosti. Članak daje cjeloviti pregled aktivnosti koje je Sveučilišna knjižnica Rijeka zajedno sa Sveučilištem u Rijeci poduzela za ostvarivanje preduvjeta za postizanje otvorenosti znanstvenih istraživanja aktivnim uključivanjem svih dionika znanstvene djelatnosti.
Ovakav prikaz dobre prakse može poslužiti drugim visokoškolskim i sveučilišnim knjižnicama kao primjer na koje sve načine mogu poduprijeti otvorenu znanost u sredini u kojoj djeluju.
The net level of immunosuppression in kidney transplant recipients is difficult to assess. QuantiFERON Monitor (QFM) is an in vitro diagnostic test that detects interferon-γ (IFN-γ) release in ...peripheral blood. The aim of our study was to compare QFM testing results in stable kidney transplant recipients and kidney transplant recipients with infection, in a single-centre cohort.We enrolled 71 kidney transplant recipients from our transplantation centre. They were divided into 2 groups according to clinical presentation (Stable kidney transplant recipients or Infection).There were no significant differences in interferon-γ release between the 2 groups (Stable kidney transplant recipients 140.59 ± 215.28 IU/ml, Infection group 78.37 ± 197.03 IU/ml, P = .24). A further analysis revealed that kidney transplant recipients presenting with bacterial infection had significantly lower IFN-γ release when compared to stable kidney transplant recipients (26.52 ± 42.46 IU/ml vs 140.59 ± 215.28 IU/ml, P = .04).Kidney transplant recipients presenting with bacterial infection had lower IFN-γ release when compared to stable kidney transplant recipients. The QFM test may be useful as a tool to help guide immunosuppression dosing in kidney transplant recipients, but further studies are required to confirm its diagnostic value.
Digital morphology analyzers are increasingly replacing light microscopy in laboratory hematology practice. This study aimed to perform the analytical validation of the white blood cell (WBC) ...differential and of reliability of platelet assessment on Sysmex DI-60 (Kobe, Japan).
Validation included determination of within-run and between-run precision for WBC differential according to the CLSI EP15-A3 protocol, accuracy and method comparison with light microscopy and with the automated WBC differential from the Sysmex XN-10 hematology analyzer, reliability of platelet clump detection and platelet count estimation.
Standard deviations of both pre- and post-classification mostly satisfied manufacturer's criteria for imprecision. Accuracy assessment revealed that only eosinophil count (1.4%) in one peripheral blood smear (PBS) remained outside the declared range (2-10%) after reclassification. Method comparison between DI-60 and light microscopy yielded Spearman's correlation coefficients from 0.37 (basophils) to 0.94 (neutrophils and lymphocytes), minor proportional difference for bands, constant difference for monocytes, both constant and proportional difference for lymphocytes and statistically significant biases for bands, lymphocytes, monocytes and basophils. Diagnostic sensitivity (Se) and specificity (Sp) of DI-60 in detecting immature/pathological cells were 88.7% (95%CI:81.1-94.0) and 83.0% (95%CI:78.7-86.7), respectively, with the area under the curve (AUC) of 0.86 (95%CI:0.82-0.89). Agreement in detection of platelet clumps was 94.8% (kappa coefficient = 0.67, 95%CI:0.53-0.80). Se and Sp of DI-60 to detect platelet clumps were 65.7% (95%CI: 47.8-80.9) and 96.9% (95%CI: 93.9-98.6), respectively, while AUC was 0.81 (95%CI: 0.76-0.86).
DI-60 provides reliable WBC differential and platelet assessment. In doubtful cases, the use of light microscopy is still mandatory.
In 2019 The Croatian Working Group for Laboratory Hematology, on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine, wanted to explore the background in field of ...laboratory hematology routine practice among Croatian laboratories in order to develop future strategies for producing national recommendations, if needed.
During April and May 2019, a comprehensive survey covering all main parts of the total testing process within the field of laboratory hematology among Croatian medical laboratories was conducted. The survey comprised 49 inquiries. Data was collected using Survey Monkey (Palo Alto, CA, USA). All collected data was anonymized.
The response rate was 72%. There is still a substantial number of laboratories that have only three-part differential hematology analyzers (9%). Furthermore, a very high number of laboratories did not perform analyzer verification prior to implementation into routine work (31%). Out of those who have verified their analyzers, a diversity of guidelines and recommendations were used. Nearly 10% of the laboratories do not have a defined policy regarding specimen rejection. The majority of the participants perform internal quality control daily (83%), however, only 51% of respondents evaluate the agreement between different hematology analyzers on daily basis. Although more than 90% of Croatian laboratories have a defined policy regarding specimen rejection, only 61% of respondents continuously monitor quality indicators in routine practice.
The survey revealed substantial differences in all aspects of laboratory hematology practices among Croatian medical laboratories, indicating the need for universal recommendations at the national level.
Blood gas analysis (BGA) is exposed to risks of errors caused by improper sampling, transport and storage conditions. The Clinical and Laboratory Standards Institute (CLSI) generated documents with ...recommendations for avoidance of potential errors caused by sample mishandling. Two main documents related to BGA issued by the CLSI are GP43-A4 (former H11-A4) Procedures for the collection of arterial blood specimens; approved standard - fourth edition, and C46-A2 Blood gas and pH analysis and related measurements; approved guideline - second edition. Practices related to processing of blood gas samples are not standardized in the Republic of Croatia. Each institution has its own protocol for ordering, collection and analysis of blood gases. Although many laboratories use state of the art analyzers, still many preanalytical procedures remain unchanged. The objective of the Croatian Society of Medical Biochemistry and Laboratory Medicine (CSMBLM) is to standardize the procedures for BGA based on CLSI recommendations. The Working Group for Blood Gas Testing as part of the Committee for the Scientific Professional Development of the CSMBLM prepared a set of recommended protocols for sampling, transport, storage and processing of blood gas samples based on relevant CLSI documents, relevant literature search and on the results of Croatian survey study on practices and policies in acid-base testing. Recommendations are intended for laboratory professionals and all healthcare workers involved in blood gas processing.
Abstract The potency assay for the freeze-dried live attenuated rubella vaccine is a cell culture based biological assay. The aim of our study was to perform the robustness testing of the rubella ...vaccine potency assay prior to validation. Seven intra-assay operating conditions that could have an effect on the assay performance were identified and their influence on the overall assay variability investigated by fractional factorial design of experiments (DoE). The robustness testing through DoE showed that the rubella vaccine potency assay is a robust assay. Critical operating conditions can be identified using DoE, which indicates that it is a suitable approach in bioassay robustness studies.
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