Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In ...this study, through whole-genome sequencing of a recombinant inbred line population derived from a cross between a wild soybean ZYD7 and a cultivated soybean HN44, and mapping of quantitative trait loci for seed weight, we discovered that a phosphatase 2C-1 (PP2C-1) allele from wild soybean ZYD7 contributes to the increase in seed weight/size. PP2C-1 may achieve this function by enhancing cell size of integument and activating a subset of seed trait-related genes. We found that PP2C-1 is associated with GmBZR1, a soybean ortholog of Arabidopsis BZR1, one of key transcription factors in brassinosteroid (BR) signaling, and facilitate accumulation of dephosphorylated GmBZR1. In contrast, the PP2C-2 allele with variations of a few amino acids at the N-terminus did not exhibit this function. Moreover, we showed that GmBZR1 could promote seed weight/size in transgenic plants. Through analysis of cultivated soybean accessions, we found that 40% of the examined accessions do not have the PP2C-1 allele, suggesting that these accessions can be improved by introduction of this allele. Taken together, our study identifies an elite allele PP2C-1, which can enhance seed weight and/or size in soybean, and pinpoints that manipulation of this allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.
By quantitative trait loci mapping, our study identified an elite allele, PP2C-1, which was found to associate with a transcription factor GmBZR1 and facilitate accumulation of dephosphorylated GmBZR1. Furthermore, the interaction between PP2C-1 and GmBZR1 could enhance seed weight/size. Manipulation of the PP2C-1 allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.
NiMo alloys are efficient electrocatalysts in alkaline water electrolyzer for the hydrogen evolution reaction (HER). Metals are usually considered to be stable during the cathodic process. However, ...the actual behaviors of Mo in the NiMo alloys are unexplored. Here, we present the instability of Mo in the Ni4Mo alloy as a highly efficient HER electrocatalyst in an alkaline medium. Mo in Ni4Mo is oxidized and dissolved in the form of MoO42− first. The dissolved MoO42− will re‐adsorb on the electrode surface and polymerize. Theoretical calculations indicate that the adsorption of the dimer Mo2O72− can promote the HER activity of metal Ni. The addition of MoO42− to the electrolyte can not only repair the durability of Ni4Mo alloy, but also facilitate the HER activity of pure metal of Ni, Fe, and Co. Our findings provide insight into the structural transformation mechanism and performance‐enhanced origin of cathodic materials under the reaction conditions.
NiMo alloys, as one of the most efficient electrocatalysts in the alkaline electrolyzer, are unstable during the alkaline hydrogen evolution. An in situ transformation including oxidative dissolution of Mo in the form of MoO42−, the re‐adsorption of MoO42−, the polymerization of MoO42− to Mo2O72−, and the activity promotion by the Mo2O72− is presented.
ABSTRACT
Adaptations of post‐hatching animals have attracted far more study than have embryonic responses to environmental challenges, but recent research suggests that we have underestimated the ...complexity and flexibility of embryos. We advocate a dynamic view of embryos as organisms capable of responding – on both ecological and evolutionary timescales – to their developmental environments. By viewing embryos in this way, rather than assuming an inability of pre‐hatching stages to adapt and respond, we can broaden the ontogenetic breadth of evolutionary and ecological research. Both biotic and abiotic factors affect embryogenesis, and embryos exhibit a broad range of behavioural and physiological responses that enable them to deal with changes in their developmental environments in the course of interactions with their parents, with other embryos, with predators, and with the physical environment. Such plasticity may profoundly affect offspring phenotypes and fitness, and in turn influence the temporal and spatial dynamics of populations and communities. Future research in this field could benefit from an integrated framework that combines multiple approaches (field investigations, manipulative experiments, ecological modelling) to clarify the mechanisms and consequences of embryonic adaptations and plasticity.
Fluorophores and probes are invaluable for the visualization of the location and dynamics of gene expression, protein expression, and molecular interactions in complex living systems. Rhodamine dyes ...are often used as scaffolds in biological labeling and turn‐on fluorescence imaging. To date, their absorption and emission spectra have been expanded to cover the entire near‐infrared region (650–950 nm), which provides a more suitable optical window for monitoring biomolecular production, trafficking, and localization in real time. This review summarizes the development of rhodamine fluorophores since their discovery and provides strategies for modulating their absorption and emission spectra to generate specific bathochromic‐shifts. We also explain how larger Stokes shifts and dual‐emissions can be obtained from hybrid rhodamine dyes. These hybrid fluorophores can be classified into various categories based on structural features including the alkylation of amidogens, the substitution of the O atom of xanthene, and hybridization with other fluorophores.
A bright road ahead for rhodamine: Rhodamine fluorophores are often used as scaffolds in biological labeling and turn‐on fluorescent imaging. To date, their absorption and emission spectra have been expanded to cover the entire near‐infrared region (650–950 nm), which has provided more suitable optical windows for monitoring biomolecular production, trafficking, and localization in real time.
In this study, we successfully synthesized two types of meso/microporous carbon materials through the carbonization and potassium hydroxide (KOH) activation for two different kinds of ...hyper-crosslinked polymers of TPE-CPOP1 and TPE-CPOP2, which were synthesized by using Friedel–Crafts reaction of tetraphenylethene (TPE) monomer with or without cyanuric chloride in the presence of AlCl3 as a catalyst. The resultant porous carbon materials exhibited the high specific area (up to 1100 m2 g−1), total pore volume, good thermal stability, and amorphous character based on thermogravimetric (TGA), N2 adsoprtion/desorption, and powder X-ray diffraction (PXRD) analyses. The as-prepared TPE-CPOP1 after thermal treatment at 800 °C (TPE-CPOP1-800) displayed excellent CO2 uptake performance (1.74 mmol g−1 at 298 K and 3.19 mmol g−1 at 273 K). Furthermore, this material possesses a high specific capacitance of 453 F g−1 at 5 mV s−1 comparable to others porous carbon materials with excellent columbic efficiencies for 10,000 cycle at 20 A g−1.
Facing the increasingly fierce market competition, enterprises need to make correct marketing decisions. Based on the decision support system, cloud server, and SOA framework, the article establishes ...an enterprise intelligent marketing decision-making system by combining the TCP/IP communication protocol and designing a data warehouse to store the marketing decision-making data. The Apriori algorithm in association rules is used to generate marketing rules to assist marketing decision-making, the marketing data is classified using the random forest algorithm, and the sparrow search algorithm optimizes the parameters of the random forest model. In practice, simulation experiments were designed to analyze the performance of the random forest model and the marketing decision-making system, and they were applied to the marketing decision-making example of the e-commerce platform. It was found that the AUC value obtained when the number of binary trees of the random forest model was 75 was 0.836, and the number of conflicts per second of the system was only 0.41 when the number of concurrent users was 5*104. The use of association rules resulted in eight marketing decisions, with the highest probability of user purchase prediction reaching 98.54%. Based on the intelligent marketing decision system, it can help decision-makers better analyze market changes and provide decision support for enterprises to develop scientific and reasonable marketing strategies.
ABSTRACT
Temperature profoundly affects the rate and trajectory of embryonic development, and thermal extremes can be fatal. In viviparous species, maternal behaviour and physiology can buffer the ...embryo from thermal fluctuations; but in oviparous animals (like most reptiles and all birds), an embryo is likely to encounter unpredictable periods when incubation temperatures are unfavourable. Thus, we might expect natural selection to have favoured traits that enable embryos to maintain development despite those fluctuations. Our review of recent research identifies three main routes that embryos use in this way. Extreme temperatures (i) can be avoided (e.g. by accelerating hatching, by moving within the egg, by cooling the egg by enhanced rates of evaporation, or by hysteresis in rates of heating versus cooling); (ii) can be tolerated (e.g. by entering diapause, by producing heat‐shock proteins, or by changing oxygen use); or (iii) the embryo can adjust its physiology and/or developmental trajectory in ways that reduce the fitness penalties of unfavourable thermal conditions (e.g. by acclimating, by exploiting brief windows of favourable conditions, or by producing the hatchling phenotype best suited to those incubation conditions). Embryos are not simply passive victims of ambient conditions. Like free‐living stages of the life cycle, embryos exhibit behavioural and physiological plasticity that enables them to deal with unpredictable abiotic challenges.
Solid fuels, an important source of severe Household Air Pollution (HAP) linked to many adverse health outcomes, has been widely consumed around the world. China consumes large amounts of solid fuels ...and suffers from serious indoor and outdoor air pollution. Though global HAP issues had been reviewed in previous literatures, peer-reviewed Chinese publications were seldom included in those reviews. We conducted a literature review on the studies of HAP and personal exposure in rural China with inputs from peer-reviewed publications in both English and Chinese. A total of 36,572 articles were retrieved, 294 were read in full text, of which 92 were included in final data extraction and in-depth analysis. Although HAP is a very serious issue in China, studies on either HAP or personal exposure assessment were very limited. From existing studies, levels of air pollutants including carbon monoxide, sulfur dioxide, particulate matter (PM), organic carbon, elemental carbon, polycyclic aromatic hydrocarbons (PAHs), etc., in indoor and ambient air were analyzed for their temporal and spatial variations, and the differences across different fuel types were compared. The studies showed that PM and PAHs levels in most rural homes exceeded the World Health Organization (WHO) and Chinese National Standards, especially during the heating season in northern China. Replacing traditional fuels with cleaner ones (such as liquid petroleum gas (LPG), biogas or electricity) was considered as the most appropriate way to mitigate HAP. The daily exposure to PM and PAHs from using LPG, biogas or electricity was considerably lower than that from using traditional solid fuels. However, the level was still higher than the guideline values for PM and PAHs set by WHO to protect human health. To achieve a more effective control, the current data gap need to be closed and suggestions for future research were discussed in this review.
Display omitted
•Still high HAP and personal exposure in rural China.•Replacing solid fuels with cleaner fuels can reduce HAP and personal exposure.•Using portable personal samplers is preferred to evaluate “true” personal exposure.•Provided several recommendations for future research on HAP in rural China.
An in-depth review to achieve a comprehensive understanding on the research status and the characteristics of household air pollution and personal exposures to various air pollutants in rural China.